Overview

Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.

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Author:   Helder Maiato (Principal Investigator/Group Leader, Chromosome Instability and Dynamics Lab, Instituto de Investigacao e Inovacao em Saude da Universidade do Porto Rua Alfredo Allen, Porto, Portugal) ,  Melina Schuh (Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Gottingen, Germany)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   1.090kg
ISBN:  

9780128141427

ISBN 10:   0128141425
Pages:   390
Publication Date:   25 June 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface Helder Maiato and Melina Schuh 1. Mitotic live-cell imaging at different timescales Anna-Maria Olziersky, Chris A. Smith, Nigel Burroughs, Andrew D. McAinsh and Patrick Meraldi 2. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture Nicholas I. Clarke and Stephen J. Royle 3. Quantification of three-dimensional spindle architecture Norbert Lindow, Stefanie Redemann, Florian Brünig, Gunar Fabig, Thomas Müller-Reichert and Steffen Prohaska 4. Quantitative live and super-resolution microscopy of mitotic chromosomes Nike Walther and Jan Ellenberg 5. Live imaging of cell division in 3D stem-cell organoid cultures Ana C.F. Bolhaqueiro, Richard H. van Jaarsveld, Bas Ponsioen, René M. Overmeer, Hugo J. Snippert and Geert J.P.L. Kops 6. Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide Iskra Yanakieva, Marija Matejcic and Caren Norden 7. Advanced microscopy methods for bioimaging of mitotic microtubules in plants Petra Vyplelová, Miroslav Ovecka, George Komis and Jozef Šamaj 8. Microsurgery and microinjection techniques in mitosis research Charles A. Day, Jessica Hornick, Alyssa Langfald, Christopher Mader and Edward H. Hinchcliffe 9. Analyzing the micromechanics of the cell division apparatus Yuta Shimamoto and Tarun M. Kapoor 10. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells Ana Milas, Mihaela Jagric, Jelena Martincic and Iva M. Tolic 11. Live imaging of C. elegans oocytes and early embryos Kimberley Laband, Benjamin Lacroix, Frances Edwards, Julie C. Canman and Julien Dumont 12. Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes Pierre Romé and Hiroyuki Ohkura 13. Methods to study meiosis in insect spermatocytes James R. LaFountain, Jr and Christopher S. Cohan 14. Live-cell microscopy of meiosis in spermatocytes Hiroki Shibuya and Yoshinori Watanabe 15. Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy Judith Reichmann, Manuel Eguren, Yu Lin, Isabell Schneider and Jan Ellenberg 16. Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos Mariia Burdyniuk, Natalia Wesolowska, Michal Fleszar, Matthia A. Karreman, Pedro Machado, Joana Borrego-Pinto, Bernhard Ruthensteiner, Yannick Schwab and Péter Lénárt 17. A microscopy-based approach for studying meiosis in live and fixed human oocytes Agata P. Zielinska and Melina Schuh 18. Superresolution imaging of the synaptonemal complex Katharina Schücker, Markus Sauer and Ricardo Benavente 19. Cryoelectron tomography of SYCP3 fibres under native conditions Daniel Bollschweiler, Laura Radu and Luca Pellegrini

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Author Information

Helder Maiato graduated in Biochemistry and holds a PhD in Biomedical Sciences from the University of Porto. He was a visiting PhD student at the University of Edinburgh (UK) and a Post-doctoral Research Affiliate at the New York State Department of Health (USA). At present, he is a Principal Investigator at the Institute for Research and Innovation in Health (i3S) and Invited Professor at the Faculty of Medicine of the University of Porto (Portugal). In 2015 he was honoured with the Young Investigator Award from the Louis-Jeantet Foundation and was recently elected EMBO Member. He served as National Counsellor for Science and Technology to the Portuguese Prime Minister and founded Yscience (www.yscience.pt), a pilot non-profit initiative that brings together scientists to promote science education in young children. His main research interest is the spatiotemporal control of chromosome segregation during cell division. Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

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