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OverviewInternationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers. Full Product DetailsAuthor: William C. Copeland (National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA)Publisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 197th ed. Volume: v. 197 Dimensions: Width: 15.20cm , Height: 3.00cm , Length: 22.90cm Weight: 0.001kg ISBN: 9780896039728ISBN 10: 0896039722 Pages: 432 Publication Date: 01 January 2002 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: Out of stock  Table of ContentsPart I. Methods for the Analysis of mtDNA Animal Models for Mitochondrial Disease Douglas C. Wallace Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases Eric A. Schon, Ali Naini, and Sara Shanske Screening for Aging-Dependent Point Mutations in mtDNA Yuichi Michikawa and Giuseppe Attardi Scanning Low-Frequency Point Mutants in the Mitochondrial Genome Using Constant Denaturant Capillary Electrophoresis Weiming Zheng, Luisa A. Marcelino, and William G. Thilly Identification of mtDNA Mutations in Human Cancer Shuji Nomoto, Montserrant Sanchez-Cespedes, and David Sidransky In Situ PCR of the Common Human mtDNA Deletion: Is It Related to Apoptosis? Steven J. Zullo Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler Alice Wong and Gino Cortopassi Yeast Nuclear Genes for mtDNA Maintenance Francoise Foury Measuring mtDNA Mutation Rates in Saccharomyces cerevisiae Using the mtArg8 Assay Micheline K. Strand and William C. Copeland Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR Janine H. Santos, Bhaskar S. Mandavilli, and Bennett Van Houten Migration of mtDNA into the Nucleus Mary K. Thorsness, Karen H. White, and Peter E. Thorsness 2D Gel Electrophoresis of mtDNA Heather E. Lorimer Part II. Methods for the Analysis of Proteins Involved in mtDNA Transactions Purification of Mitochondria for Enzymes Involved in Nucleic Acid Transactions Daniel F. Bogenhagen Purification of Mitochondrial Uracil DNA Glycosylase Using Ugi Sepharose Affinity Chromatography Samuel E. Bennett, Mary Jane Shroyer, Jung-Suk Sung, and Dale W. Mosbaugh Characterization of Specialized mtDNA Glycosylases Simon G. Nyaga and Vilhelm A. Bohr Purification, Separation, and Identification of the Human mtDNA Polymerase With and Without Its Accessory Subunit Matthew J. Longley and William C. Copeland Assay of mtDNA Polymerase g from Human Tissues Robert K. Naviaux Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase g Carol L. Farr and Laurie S. Kaguni Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: Mitochondrial Single-Stranded DNA-Binding Protein Carol L. Farr and Laurie S. Kaguni Expression, Purification and In Vitro Assays of Mitochondrial Single-Stranded DNA-Binding Protein Kang Li and R. Sanders Williams Recombinant Yeast mtDNA Helicases: Purification and Functional Assays Silja Kuusk, Tiina Sedman, and Juhan Sedman Isolation of DNA Topoisomerases I and II Activities from Mitochondria of Mammalian Heart Robert L. Low Endonuclease G Isolation and Assays Robert L. Low and Mariana Gerschenson Targeting DNA Repair Proteins to Mitochondria Allison W. Dobson, Mark R. Kelley, Glenn L. Wilson, and Susan P. LeDoux In Organello Footprinting of mtDNA Steven C. Ghivizzani, Cort S. Madsen, Chandramohan V. Ammini, and William W. Hauswirth Crosslinking of Proteins to mtDNA Brett A. Kaufman, Scott M. Newman, Philip S. Perlman, and Ronald A. Butow Breeding and Genotyping of Tfam Conditional Knockout Mice Mats Ekstrand and Nils-Goran LarssonReviewsAccordingly, the book is timely and of significant use to investigators working in this area. The book is divided into two major sections -- the first deals with analysis of the DNA and the second with analysis of related proteins. A valuable introductory chapter discusses available animal models for mitochondrial disease and the book concludes with a discussion of knockout mice. Given the general interest in mitochondrial mutations as they relate to a variety of human diseases, this contribution is timely and of value. The protocols are sufficiently detailed to allow adaptation into any working laboratory. - Doody's Health Sciences Book Review Journal Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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