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OverviewFocused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout. Full Product DetailsAuthor: Niall BarronPublisher: Springer Imprint: Springer Edition: 2012 ed. Dimensions: Width: 15.50cm , Height: 0.70cm , Length: 23.50cm Weight: 0.221kg ISBN: 9789400795266ISBN 10: 9400795262 Pages: 126 Publication Date: 14 December 2014 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsPreface.-1 A general introduction to microRNAs, their investigation and exploitation in CHO cell lines.-2 Biogenesis of Mammalian miRNA.-3 Target prediction algorithms and bioinformatics resources for miRNA studies.-4 The CHO miRNA transcriptome.-5 MicroRNAs as engineering targets - pathway manipulation to impact bioprocess phenotypes.-6 MicroRNAs for Enhancement of CHO Cell Proliferation and Stability Insights from Neuroblastoma Studies.-7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production.-8 Manipulating Levels of Specific microRNAs in Mammalian Cells.- Index.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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