Overview

The technique of needle microinjection has become a prominent experimental approach in biological research. Cellular organelles, DNA and RNA, enzymes, structural proteins, metabolites, ions and antibodies are just some of the molecular and cellular elements that have been transposed from test tubes into living cells by needle injection. This technique is broadly used as a valuable tool for the study of many different cell responses in a variety of systems. This text presents information on the technique of needle microinjection's principles, the required equipment, preparation of receiving cells and injected material and the analysis of the results. It provides detailed protocols for a wide range of important applications and different cellular systems from the fields of cell cycle regulation, signal transduction, study of transcriptional regulations, cytoskeletal functions, secretion and intracellular transport.

Full Product Details

Author:   J.C. Lacal ,  etc.
Publisher:   Birkhauser Verlag AG
Imprint:   Birkhauser Verlag AG
Edition:   New edition
ISBN:  

9783764359737

ISBN 10:   3764359730
Pages:   350
Publication Date:   February 1999
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Needle microinjection - a brief history, J. Feramisco, R. Perona and J.C. Lacal; microinjection of macromolecules into mammalian cells in culture - R. Perona, F. Dolfi, J. Feramisco and J.C. Lacal; the use of plasmid microinjection to study specific cell cycle phase transitions, E.S. Knudsen; combining microinjection with microanalytical methods to investigate regulation of metabolic pathways, J. Manchester and J.C. Lawrence Jr; import of stably folded proteins into peroxisomes, P.A. Walton; study of the function of microtubule proteins, R. Armas-Portela and J.Avila; cytoskeleton regulation by Rho proteins, R. Kozma, S. Ahmed and L. Lim; cytoskeletal regulation by Rho-p21 associated kinases - analysis of ROK and PAK function by plasmid microinjection, T. Leung, E. Manser and L. Lim; use of microinjection to study apoptosis and its prevention, F. Kohlhuber, H.Hermeking and D. Eick; role of the InsP3 receptor in intracellular Ca2+ release and Ca2+ entry, R.S. Mathias and H.E. Ives; microinjection of xenopus laevis oocytes - a model system, J.C. Lacal; xenopus laevis oocytes as a model for studying the activation of intracellular kinases, J.C. Lacal and A. Carnero; signal transduction in xenopus laevis oocytes by Ras oncoproteins and lipid metabolites, A. Carnero and J.C. Lacal; the multiple roles of mos during meiosis, M.S. Murakami and G.F. Vande Woude; use of the xenopus oocytes system to study RNA transport, L.A. Allison; functional expression of G protein-coupled receptors in xenopus laevis oocytes, P. De La Pena and F. Barros; expression of glucose transporters in xenopus laevis oocytes - applications for the study of membrane transporter function and regulation, M.J. Seatter and G.W. Gould; DNA injection into xenopus laevis embryos as a tool to study spatial gene activity, M. Kuhl, M. Walter, J. Clement, H. Friedle, D. Wedlich, and W. Knochel; transformation of nematodes by microinjection, S. Hashmi, G. Hashmi and R. Gaugler.

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