Overview

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.

Full Product Details

Author:   Olga Garaschuk ,  Alexei Verkhratsky
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2034
Weight:   0.669kg
ISBN:  

9781493996605

ISBN 10:   1493996606
Pages:   340
Publication Date:   14 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Microglia in Health and Disease   1. Microglia: The Neural Cells of Non-Neural Origin             Olga Garaschuk and Alexei Verkhratsky   2. Morphology of Microglia across Contexts of Health and Disease             Julie C. Savage, Micaël Carrier, and Marie-Ève Tremblay   3. Physiology of Microglia             Olga Garaschuk and Alexei Verkhratsky   4. Microglia Reactivity: Heterogeneous Pathological Phenotypes             Hélène Hirbec, François Rassendren, and Etienne Audinat   5. Microglia in Neurodegenerative Disorders             Darío Tejera and Michael T. Heneka   6. Physiological Implications of Microglia-Synapse Interactions             Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, and Junichi Nabekura   Part II: In Vitro Analyses of Microglia   7. Ultrastructural Analyses of Microglial Interactions with Synapses             Marie-Ève Tremblay and Ania K. Majewska   8. Visualizing Dark Microglia             Marie-Kim St-Pierre, Maude Bordeleau, and Marie-Ève Tremblay   9. Electrophysiological Investigation of Microglia             Elena Avignone, Giampaolo Milior, Isabelle Arnoux, and Etienne Audinat   10. Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia             Annette Masuch and Knut Biber   11. Assessment of Parasite-Microglia Interactions In Vitro             Katherine Figarella, Nestor L. Uzcategui, and Michael Duszenko   Part III: Studying Microglia In Vivo   12. In Vivo Visualization of Microglia Using Tomato Lectin             Bianca Brawek, Maria Olmedillas del Moral, and Olga Garaschuk   13. Long-Term In Vivo Imaging of Individual Microglial Cells             Angelos A. Skodras, Jasmin Hefendehl, and Jonas J. Neher   14. Analysis of Signaling Mechanisms Regulating Microglial Process Movement             Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, and Christian Madry   15. Measuring Microglial Turnover in the Adult Brain             Diego Gomez-Nicola, Gemma L. Fryatt, and Katharine E. Askew   16. Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor             Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, and Ari Waisman   17. Single Cell Electroporation for Measuring In Vivo Calcium Dynamics in Microglia             Bianca Brawek and Olga Garaschuk   18. Labeling Microglia with Genetically-Encoded Calcium Indicators             Yajie Liang and Olga Garaschuk   19. Calcium Imaging of Microglial Network Activity in Stroke             Petr Tvrdik, Kathryn N. Kearns, Khadijeh A. Sharifi, M. Filip Sluzewski, Scott T. Acton, and M. Yashar S. Kalani   20. In Vivo Two Photon Imaging of Microglial Synapse Contacts             Daisuke Kato, Ako Ikegami, Hiroshi Horiuchi, Andrew J. Moorhouse, Junichi Nabekura, and Hiroaki Wake   21. In Vivo Phagocytosis Analysis of Amyloid Beta             Darío Tejera and Michael T. Heneka   22. Functional Analysis of Brain-Engrafted Monocytes after Microglial Ablation in Mouse Models             Nicholas H. Varvel, Richard M. Ransohoff, and Jonas J. Neher   Part IV: Omics Analyses of Microglia   23. Analysis of the Microglial Sensome             Suzanne E. Hickman and Joseph El Khoury   24. Transcriptomics and Live Imaging to Define Functional Phenotypes of Microglia in Pathological Human Tissue             Giampaolo Milior, Farah Chali, Tiffany Dos Santos, Juliette Royer, Richard Miles, and Mélanie Morin-Brureau

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