Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Author:   Matthieu Piel ,  Daniel Fletcher ,  Junsang Doh
Publisher:   Elsevier Science Publishing Co Inc
ISBN:  

9780128142844


Pages:   264
Publication Date:   28 November 2018
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis


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Author:   Matthieu Piel ,  Daniel Fletcher ,  Junsang Doh
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.680kg
ISBN:  

9780128142844


ISBN 10:   0128142847
Pages:   264
Publication Date:   28 November 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Section 1 - Microfluidics for cell culture, cell manipulation and cell sorting Viable cell culture in PDMS-based microfluidic devices Melikhan Tanyeri and Savas Tay High-throughput microfluidic single-cell trapping arrays for biomolecular and imaging analysis Xuan Li and Abraham P. Lee Artificial niche microarrays for identifying extrinsic cell-fate determinants Samy Gobaa, Raphael V. Gayet and Matthias P. Lutolf Reconstruction of directed neuronal networks in a microfluidic device with asymmetric microchannels Josquin Courte, Renaud Renault, Audric Jan, Jean-Louis Viovy, Jean-Michel Peyrin and Catherine Villard Single-cell 3D electro-rotation Liang Huang, Peng Zhao, Fei Liang and Wenhui Wang Section 2 - Droplet-based microfluidics Direct quantification of EGFR variant allele frequency in cell-free DNA using a microfluidic-free digital droplet PCR assay Benjamin Demaree, Daniel Weisgerber, Ata Dolatmoradi, Makiko Hatori and Adam R. Abate Quantifying phenotypes in single cells using droplet microfluidics Fengjiao Lyu, Lucas R. Blauch and Sindy K.Y. Tang Modular microfluidics for double emulsion formation Bryant Thompson, Nareh Movsesian, Christine Cheng, Prathamesh Karandikar, Malancha Gupta and Noah Malmstadt Universal anchored-droplet device for cellular bioassays Gabriel Amselem, Sebastien Sart and Charles N. Baroud Section 3 - Section 3 - Microfluidics for cellular analysis Cell biology at the interface of nanobiosensors and microfluidics Nikhil Bhalla, Hung-Ju Chiang and Amy Q. Shen BET-seq: Binding energy topographies revealed by microfluidics and high-throughput sequencing Arjun K. Aditham, Tyler C. Shimko and Polly M. Fordyce

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Author Information

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell's environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development. Dr. Fletcher and his team develops diagnostic technologies and studies mechanical regulation of membrane and cytoskeleton organization in the context of cell motility, signaling, and host-pathogen interactions. His lab specialize in development of optical microscopy, force microscopy, and microfluidic technologies to understand fundamental organizational principles through both in vitro reconstitution and live cell experiments. Recent work includes investigating the mechano-biochemistry of branched actin network assembly with force microscopy, studying membrane deformation by protein crowding and oligomerization with model membranes, and reconstituting spindle scaling in encapsulated cytoplasmic extracts. The long-term goal of his work is to understand and harness spatial organization for therapeutic applications in cancer and infectious diseases. Junsang Doh is an associate professor of Mechanical Engineering/Interdisciplinary Bioscience and Bioengineering (I-Bio) in POSTECH, South Korea. Prof. Doh's group develops and utilizes engineering tools such as microfabrication/imaging/mechanics to study fundamental aspects of immune cell behaviors, including synapse-based cell-cell interactions and motility under complex microenvironments, in the context of cancer immunotherapy.

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