Microarrays: Volume I: Synthesis Methods

Author:   Jang B. Rampal
Publisher:   Humana Press Inc.
Edition:   2nd ed. 2007
Volume:   381
ISBN:  

9781588295897


Pages:   452
Publication Date:   27 July 2007
Format:   Hardback
Availability:   In Print   Availability explained
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Microarrays: Volume I: Synthesis Methods


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Overview

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments. Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology. Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

Full Product Details

Author:   Jang B. Rampal
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2007
Volume:   381
Dimensions:   Width: 15.20cm , Height: 2.50cm , Length: 22.90cm
Weight:   0.869kg
ISBN:  

9781588295897


ISBN 10:   1588295893
Pages:   452
Publication Date:   27 July 2007
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Current Microarray Surface Chemistries.- Nonfouling Surfaces.- Optimization of Oligonucleotide DNA Microarrays.- Detection of DNA Copy Number Alterations in Complex Genomes Using Array Comparative Genomic Hybridization.- Evaluating the Quality of Data From Microarray Measurements.- Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents.- Choice of Polymer Matrix, Its Functionalization and Estimation of Functional Group Density for Preparation of Biochips.- Methods in High-Resolution, Array-Based Comparative Genomic Hybridization.- Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression Analysis.- Construction of In Situ Oligonucleotide Arrays on Plastic.- Detecting Ligated Fragments on Oligonucleotide Microarrays.- Detection of Single-Nucleotide Polymorphisms in Cancer-Related Genes by Minisequencing on a Microelectronic DNA Chip.- Hybridization Analysis Using Oligonucleotide Probe Arrays.- In Situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing.- Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays.- Printing Low Density Protein Arrays in Microplates.- Forward-Phase and Reverse-Phase Protein Microarray.- Cell Microarray for Functional Exploration of Genomes.- Quantification of Mixed-Phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence.- Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays.- Nanotechnology.

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