Methods in Gene Technology, Volume 2

Author:   J.W. Dale (Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England) ,  P.G. Sanders (Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England)
Publisher:   Elsevier Science & Technology
Volume:   v. 2
ISBN:  

9781559382649


Pages:   370
Publication Date:   01 March 1995
Format:   Hardback
Availability:   Out of stock   Availability explained
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Methods in Gene Technology, Volume 2


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Author:   J.W. Dale (Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England) ,  P.G. Sanders (Molecular Microbiology Group, School of Biological Sciences, University of Surrey, Guildford, England)
Publisher:   Elsevier Science & Technology
Imprint:   JAI Press Inc.
Volume:   v. 2
Weight:   0.760kg
ISBN:  

9781559382649


ISBN 10:   1559382643
Pages:   370
Publication Date:   01 March 1995
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock   Availability explained
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Contents. List of Contributors. Introduction. Purification of DNA (J.W. Dale). Electrophoretic Separation of Nucleic Acids (P.G. Sanders0. Preparative Gel Electrophoresis (J.C. Maule). Plasmid Isolation Procedures (A.I. Knight). Plasmid Isolation: column Methods (T.J. Hellyer0. Isolation of Large Plasmids (G.L. Jones and P.A. Williams). Isolation of Linear and Single-Strand Plasmid DNA (J.C. Alonso). Preparation of Bacteriophage &lgr; DNA (E. Norman). Purification of Viral RNA (J.E. Whitby, A.D.T. Barrett, A.D. Jennings, and P. Johnstone). Large Scale DNA Sequencing by Manual Methods (A.J. Davison and E.A.R. Telford). Pulsed-Field Gel Electrophoresis: Applications to Bacteria (P.R. Stewart, W. El-Adhami, and B. Inglis). Pulsed-Field Gel Electrophoresis: Large Scale Restriction Maps (N. Okahashi and T. Koga). Pulsed-Field Gel Electrophoresis: Isolation and Analysis of Large Linear Plasmids (H. Kinashi). Detection of Mutations by PCR-Single-Strand Conformation Polymorphism (A. Telenti). Analysis of DNA Supercoiling (K. Drlica, M. Malik, J.Y. Wang, A. Sasiak and R.R. Sinden). Gel Retardation Assays and DNA Footprinting (I.S. Goping, D.A. Underhill, and G.C. Shore). Southwestern Blot (G. Corte and M.T. Corsetti). Genomic Fingerprinting Using the PCR-Random Amplified Polymorphic DNA Technique (S.V.P.S. Silva and J.D. Procunier). PCR Walking (M.G. Burdon and J.J. Willoughby). Index.

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