Overview

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

Full Product Details

Author:   Juan C. Fontecilla-Camps ,  Yvain Nicolet
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2014 ed.
Volume:   1122
Dimensions:   Width: 17.80cm , Height: 2.30cm , Length: 25.40cm
Weight:   7.318kg
ISBN:  

9781627037938

ISBN 10:   1627037934
Pages:   299
Publication Date:   18 March 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Advances in Research on Metalloproteins.- Purification of O2-Sensitive Metalloproteins.- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources.- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins.- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers.- Electrochemistry of Metalloproteins – Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1.- FTIR Spectroscopy of Metalloproteins.- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy.- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds.- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR).- Mössbauer Spectroscopy.- X-Ray Absorption Spectroscopy of Metalloproteins.- X-Ray Crystallographic Studies of Metalloproteins.- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds.- Density Functional Theory – Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.

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