Overview

This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-products, detect enzyme and kinase activities, detecting a variety of metabolic pathways, and intracellular networks including nucleotide metabolism and the metabolic response to hypoxia and cytotoxic drugs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Metabolic Reprogramming: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Full Product Details

Author:   Salvatore Papa ,  Concetta Bubici
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2675
Weight:   0.845kg
ISBN:  

9781071632468

ISBN 10:   1071632469
Pages:   330
Publication Date:   01 June 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.             In Vivo Tissue Lipid Uptake in Antisense Oligonucleotides (ASO) Treated Mice Igor Aurrekoetxea, Beatriz Gomez-Santos, Maider Apodaka-Biguri, Mikel Ruiz de Gauna, Francisco Gonzalez-Romero, Xabier Buqué, and Patricia Aspichueta   2.             In Vivo Hepatic Triglyceride Secretion Rate in Antisense Oligonucleotides (ASO) Treated Mice Beatriz Gomez-Santos, Diego Saenz de Urturi, Xabier Buqué, Igor Aurrekoetxea, Ane Nieva, Idoia Fernández-Puertas, and Patricia Aspichueta   3.             Measurement of Fatty Acid Oxidation by High Resolution Respirometry: Special Considerations for Analysis of Skeletal and Cardiac Muscle and Adipose Tissue. Nicole T. Watt, Amanda DV. MacCannell, and Lee D. Roberts.   4.             In Vivo Imaging of Bone Marrow Long Chain Fatty Acid Uptake Jayna J. Mistry and Stuart A. Rushworth   5.             Quantification and Tracing of Stable Isotope into Cysteine and Glutathione Yun Pyo Kang and Gina M. DeNicola   6.             Targeted Quantification of Amino Acids by Dansylation Yuanyuan Liu, Haoqing Chen, and Dylan Dodd   7.             Isolation of Mitochondria from Mouse Tissues for Functional Analysis Rebeca Acín-Pérez, Katrina P. Montales, Kaitlyn B. Nguyen, Alexandra J. Brownstein, Linsey Stiles, and Ajit S. Divakaruni   8.             Methods for Monitoring Mitochondrial Biogenesis and Turnover in Cultured Hepatocytes and Mouse Liver Using MitoTimer Reporter Assay Xiaowen Ma, Wen-Xing Ding   9.             Quantification of Intracellular ATP Content in Ex-Vivo GC B Cells Marta Iborra Pernichi, Jonathan Ruiz García, and Nuria Martínez-Martín   10.         UHPLC-HRMS-based Analysis of S-Hydroxymethyl-Glutathione, GSH and GSSG in Human Cells María Eugenia Monge, Manuela R. Martinefski, Mariela Bollini and Lucas B. Pontel   11.         Quantitation of glutathione and oxidized glutathione ratios from biological matrices using LC-MS/MS Thomas P. Mathews   12.         Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells. Gaetano Calabrese, Lianne J.H.C. Jacobs, and Jan Riemer   13.         Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue Raquel Saborano, Emma Shepherd, Ulrich L. Günther and Patricia F. Lalor   14.         13C isotope labeling and mass spectrometric isotope enrichment analysis in acute brain slices Elisa Motori and Patrick Giavalisco   15.         Metabolite analyses using Nuclear Magnetic Resonance (NMR) spectroscopy in plasma of patients with prostate cancer Dalia Ahmed, Stefano Cacciatore, and Luiz Fernando Zerbini   16.         Screening Kinase-Dependent Phosphorylation of Key Metabolic Reprogramming Regulators Fatma Necmiye Kaci, Alessio Lepore, Salvatore Papa, and Concetta Bubici   17.         Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPDH) Claudia Montllor-Albalate, Anna E. Thompson, Hyojung Kim and Amit R. Reddi   18.         Quantitation of Glutamine Synthetase-1 Activity in Drosophila Melanogaster. Teresa Vitali, Maria Antonietta Vanoni, and Paola Bellosta   19.         Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation Dilem Shakir, Michael Batie, and Sonia Rocha   20.         Metabolic Reprogramming During B Cell Differentiation Sophie Stephenson and Gina M. Doody   21.         An Integrated Methodology to Quantify the Glycolytic Stress in Plasma Cell Myeloma in Response to Cytotoxic Drugs Alessio Lepore, Fatma Necmiye Kaci, Concetta Bubici, and Salvatore Papa   22.         Spatial Analysis of Nucleotide Metabolism: From CRISPR Knock Out Cancer Cells to MALDI Imaging of Tumors Petra Hyrossova, Mirko Milosevic, Ahmad Y. Alghadi, Lukas Kucera, Jan Prochazka, Radislav Sedlacek, Jakub Rohlena, and Katerina Rohlenova   23.         Assessment of Metabolic Pathways and Parameters in Extracellular Matrix-Detached Cells Lisa M. Hom and Zachary T. Schafer   24.         Metabolic Networks: Weighted Gene Correlation Network Analysis Lise Desquilles and Orlando Musso

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