Overview

This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.

Full Product Details

Author:   Charles G. Cranfield
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2402
Weight:   0.596kg
ISBN:  

9781071618455

ISBN 10:   1071618458
Pages:   300
Publication Date:   03 December 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Methods for Forming Giant Unilamellar Fatty Acid Vesicles             Lauren A. Lowe, Daniel W.K. Loo, and Anna Wang   2. Preparing Ion Channel Switch Membrane-Based Biosensors             Amani Alghalayini, Charles G. Cranfield, Bruce A. Cornell, and Stella M. Valenzuela   3. Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer             Charles G. Cranfield, Anton Le Brun, Alvaro Garcia, Bruce A. Cornell, and Stephen A. Holt   4. Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes             Tadas Penkauskas, Filipas Ambrulevičius, and Gintaras Valinčius   5. Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes             Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster   6. Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs)             Hadeel Alobeedallah, Bruce A. Cornell, and Hans Coster   7. Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes             Lissy M. Hartmann, Alvaro Garcia, Evelyne Deplazes, and Charles G. Cranfield   8. De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer             Kan Shoji   9. Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations             Sheikh I. Hossain, Mohammad Z. Islam, Suvash C. Saha, and Evelyne Deplazes   10. Establishing a Lipid Bilayer for Molecular Dynamics Simulations             Robby Manrique   11. Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins             Amanda Buyan and Ben Corry   12. Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling             Kathleen Wood   13. Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation             Michael H.L. Nguyen, Mitchell DiPasquale, Stuart R. Castillo, and Drew Marquardt   14. Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering             Mitchell DiPasquale, Michael H.L. Nguyen, Stuart R. Castillo, Frederick A. Heberle, and Drew Marquardt   15. Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers             Stephen A. Holt, Tara E. Oliver, and Andrew R.J. Nelson   16. Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs)             Kinga Burdach, Damian Dziubak, and Slawomir Sek   17. Manipulation of Lipid Membranes with Thermal Stimuli             Karolina Spustova, Lin Xue, Ruslan Ryskulov, Aldo Jesorka, and Irep Gözen   18. Analyzing Morphological Properties of Early-Stage Toxic Amyloid β Oligomers by Atomic Force Microscopy             Dusan Mrdenovic, Jacek Lipkowski, and Piotr Pieta   19. Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains             Romina F. Vázquez, Erasmo Ovalle-García, Armando Antillón, Iván Ortega-Blake, Carlos Muñoz-Garay, and Sabina M. Maté   20. Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR             Elvis Pandzic, Renee Whan, and Alex Macmillan   21. Bacterial Dye Release Measures in Response to Antimicrobial Peptides             Srikanth Dumpati and Debarun Dutta   22. Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi             Maria Makarova and Dylan M. Owen

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