Overview

Leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories.

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Reviews

The editor, Ian M. Clark, has done an excellent job in putting together a collection of articles that span what is now a fairly wide field. the content and writing are of a high standard In chapter 3, covering structural studies and contributed by Wolfram Bode and Klaus Maskos, the authors point out the absence of specific discussions of three-dimensional structures amongst the plethora of general reviews on MMPs and TIMPs. Their contribution expertly fills that void. Indeed the Notes' section, at the end of each chapter, is where the benefit of much, often hard-earned, experience is to be reaped and is a valuable addition in almost every case. The last two sections, between them, cover detection of MMp and TIMP expression at both RNA and protein levels, and actively assays. Hore, particular mention must be made of the chapter dealing with zymography and reverse zymography in which Susan Hawkes and coauthors have carefully explained this technique. Their use of figures with detailed interpretation is really excellent and alone could justify the purchase of this book for many labs. this book is recommended as a reference text for all labs engaged in MMP research. There is enough variety for established research labs to find some new methods, and, for newcomers to the field, it should make working with MMPs and their inhibitors a very straightforward prospect. - TRENDS in Cell Biology

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