Master's Thesis I: Function-Analysis of Seventeen Function-Unknown Genes in Bacillus Subtilis

Author:   Widad Akrawi, Dr ,  Dr Widad Akrawi
Publisher:   Grin Verlag
ISBN:  

9783656469025


Pages:   162
Publication Date:   05 August 2013
Format:   Paperback
Availability:   In stock   Availability explained
We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately.

Our Price $192.46 Quantity:  
Add to Cart

Share |

Master's Thesis I: Function-Analysis of Seventeen Function-Unknown Genes in Bacillus Subtilis


Add your own review!

Overview

Master's Thesis from the year 1998 in the subject Biology - Genetics / Gene Technology, grade: 11 on 13-scale, - (Technical University of Denmark - Microbiology), language: Danish, comment: Widad Akrawi received her Master's degree in genetics and microbiology in 1999 from the Technical University of Denmark, which is the best technical University in Scandinavia and has been ranked the 5th in Europe and the 69th in the World. Her dissertation was part of the European Union-funded Bacillus Subtilis Genome Sequencing Project. In her Master's Thesis I, she analyzed the function and biological roles of 17 newly sequenced genes by selectively and specifically generating more than 40 bacterial strains with mutations in the 17 function-unknown genes. See also her Master's Thesis II., abstract: Dette project handlede om en sekvens pa 22,62 kb, som indeholder 17 nyligt sekventeret gener i Bacillus subtilis, h.h.v. yut M, yut K, yut J, yut I, yut H, yut G, yut F, yut E, yut D, yut C, yunA, yun B, yunC, yunC, yunE, yunF, og yunG. Formalet med projektet var forst at mutagenisere disse ukendte gener, som skulle dernaest analyseres og sammenlignes med vildtypen. Mutationerne blev udfort ved at indfore plasmidet pMUTIN4 efter en standardiserede metode udviklet for B. Subtilis Genomprojektet. Der blev konstrueret over 56 stammer med defekter i 15 af de aktuelle gener. Stammerne indeholdte lacZ i starten af generne, hvilket muliggjorde at undersoge, hvornar generne blev udtrykt, ved blandt andet udpladning pa LB-plader med og uden IPTG. Mutant stammerne blev dernaest ved en southern blot analyse undersogt for, om de havde en eller flere kopier af plasmidet inkorporeret pa kromosomalt DNA. Southern blottet indikerede, at inkorporeringen af plasmidet var som onsket (en kopi) i 14 mutant stammer, imens der var en mutant stamme af yutJ med tandem insert. Derudover de gentagne mislykkede transformations forsog (10 forsog) for yunA og yunG understregede at disse to gener var essentielle

Full Product Details

Author:   Widad Akrawi, Dr ,  Dr Widad Akrawi
Publisher:   Grin Verlag
Imprint:   Grin Verlag
Dimensions:   Width: 14.80cm , Height: 0.90cm , Length: 21.00cm
Weight:   0.218kg
ISBN:  

9783656469025


ISBN 10:   3656469024
Pages:   162
Publication Date:   05 August 2013
Audience:   General/trade ,  General
Format:   Paperback
Publisher's Status:   Active
Availability:   In stock   Availability explained
We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately.
Language:   Danish

Table of Contents

Reviews

Author Information

Widad Akrawi is a geneticist and global health expert, and known for being a Danish human rights defender, peace advocate, and author. She was born 1969 in Akre, Kurdistan region. She graduated as a civil engineer in 1990, and proceeded on to the Technical University of Denmark where she earned a Masters degree in genetics and microbiology. After graduation, she served as a clinical geneticist and researcher in human genetics and inherited diseases at the Danish Royal Hospital, the largest hospital in Denmark and a leading university hospital in Northern Europe. She has also earned a PhD degree in global health and epidemiology. In 2003, she became a published writer. After researching the concept of victim psychology and the effects of various types of trauma on individuals' psychological health, she published her first novel, Tara's Book, written in Danish, in Copenhagen. The book was translated to Norwegian in 2005, published in Oslo, and distributed by the Norwegian Cultural Council to almost all Norwegian libraries. Her eBook, entitled Under Habets Skyggee (Under the Shadow of Hope), also in Danish, was published in 2013 at Amazon.com and Lulu.com. She currently leads Defend International, a Norway-based NGO that is devoted to medical research, and human rights. Over the years, she has addressed and actively participated in several regional and international meetings and conferences, such as the United Nations Third and Fourth Biennial Meeting of States, the UN Commission on The Status of Women, the General Assembly Interactive Hearings with Non-Governmental organizations, Civil society organizations and the Private sector, and the UN First Committee on Disarmament and International Security. She has held numerous leadership positions in national and international organizations, including executive board member of Amnesty International Denmark, ambassador for Amnesty International's Stop Torture campaign, and member of the Panel of Vestby Experts on approaches to socio-cultural constructivism. At present, she is a board member of Women of Europe Award, an active member of International Action Network on Small Arms (IANSA), a board member of IANSA Women's Network Working Group, and a senior lobbyist at the UN, lobbing for the adoption of an international Arms Trade Treaty during the negotiations that take place at the UN General Assembly's First Committee on Disarmament and International Security.

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions
Latest Reading Guide

Aorrng

Shopping Cart
Your cart is empty
Shopping cart
Mailing List