Overview

This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of mammary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast cancer. Chapters detail processes used to characterize stem cells, single cell RNA sequencing, computational methods, sophisticated imaging techniques, and a variety of model systems, among others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols, Second Edition aims to make available protocols used to navigate the intricate behavior of mammary stem cells and to gain further knowledge to take us closer to the design of innovative strategies to prevent and treat breast cancer.

Full Product Details

Author:   Maria dM. Vivanco
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2471
Weight:   0.699kg
ISBN:  

9781071621950

ISBN 10:   1071621955
Pages:   354
Publication Date:   17 February 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.     Protocol for studying embryonic mammary gland branching morphogenesis ex vivo Qiang Lan, Jyoti Satta, Satu-Marja Myllymäki, Ewelina Trela, Riitta Lindström, Beata Kaczyńska, Johanna Englund, and Marja L. Mikkola 2.     Multidimensional fluorescence imaging of embryonic and postnatal mammary gland development Claudia Carabaña and Bethan Lloyd-Lewis 3.     Single-cell transcriptomic and epigenetic analyses of mouse mammary development starting with the embryo Zhibo Ma, Nikki K. Lytle, Cynthia Ramos, Razia F. Naeem, Geoffrey M. Wahl 4.     Computational methods to identify cell fate determinants, identity transcription factors and niche induced signaling pathways for stem cell research Muhammad Ali, Mariana Messias Ribeiro, and Antonio del Sol 5.     Differential proteomic analysis of complex mixtures by label free nLC MS/MS Iraide Escobés, Mikel Azkargorta, Ibon Iloro and Felix Elortza 6.     Orthotopic transplantation of mouse mammary epithelial cells Marisa M. Faraldo, Marina A. Glukhova, and Marie-Ange Deugnier 7.     Lineage tracing methods to study mammary epithelial hierarchies in vivo Verónica Rodilla and Silvia Fre 8.     Lentiviral transduction of mammary epithelial cells Richard Iggo 9.     Modification of Single Cells within Mouse Mammary Gland Derived Acini via Viral Transduction Lucia Garcia del Valle, Marta G. Montero, and Martin Jechlinger 10.  Surgical Procedure for Implantation of Human Tumor Tissue into the Epithelium-free Mammary Fat Pad of Immunocompromised Mice to Generate Patient-Derived Xenografts (PDX) Lacey E. Dobrolecki and Michael T. Lewis 11.  The Chicken Embryo Chorioallantoic Membrane (CAM); a Versatile Tool for the Study of Patient-Derived Xenografts Hugo Villanueva and Andrew G. Sikora 12.  Intraductal injections into the mouse mammary gland Erik Oliemuller, Richard Newman, and Beatrice A. Howard 13.  Modelling breast cancer in organoid and intraductal models Richard Iggo 14.  Single organoid droplet-based staining method for high-end 3D imaging of mammary organoids Jakub Sumbal and Zuzana Koledova 15.  Divide and Conquer: Isolating Cell Populations to Investigate how Breast Cancer Risk Factors Alter the Breast Microenvironment Lisa M. Arendt 16.  An organotypic assay to study epithelial-fibroblast interactions in human breast Jakub Sumbal, Thorarinn Gudjonsson, Gunnhildur Asta Traustadottir, and Zuzana Koledova 17.  Patient-derived explant cultures of normal and tumour human breast tissue Isabel Gris-Cárdenas, Miriam Rábano, and Maria dM. Vivanco 18.  Negative-Selection Enrichment of Circulating Tumor Cells from Peripheral Blood Using the Microfluidic CTC-iChip Risa Burr, Jon F. Edd, Brian Chirn, Avanish Mishra, Daniel Haber, Mehmet Toner, and Shyamala Maheswaran 19.  Measuring Mechanical Properties of Breast Cancer Cells with Atomic Force Microscopy Barbara Zbiral, Andreas Weber, and José L. Toca-Herrera 20.  Breast cancer stem cells: a clinician´s view José Schneider and Francisco J. Vizoso  

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