Mammalian Oocyte Regulation: Methods and Protocols

Author:   Hayden A. Homer
Publisher:   Humana Press Inc.
Edition:   2013 ed.
Volume:   957
ISBN:  

9781627031905


Pages:   311
Publication Date:   09 November 2012
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Mammalian Oocyte Regulation: Methods and Protocols


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Author:   Hayden A. Homer
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2013 ed.
Volume:   957
Dimensions:   Width: 17.80cm , Height: 2.30cm , Length: 25.40cm
Weight:   7.611kg
ISBN:  

9781627031905


ISBN 10:   1627031901
Pages:   311
Publication Date:   09 November 2012
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Studying Recombination in Mouse Oocytes.- Analysis of Recombinants in Female Mouse Meiosis.- Studying Meiosis Specific Cohesins in Mouse Embryonic Oocytes.- Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development.- Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos.- Time-Lapse Epi-Fluorescence Imaging of Expressed cRNA to Cyclin B1 for Studying Meiosis I in Mouse Oocytes.- Using FRET to Study RanGTP Gradients in Live Mouse Oocytes.- Making cRNA for Microinjection and Expression of Fluorescently-Tagged Proteins for Live-Cell Imaging in Oocytes.- RNAi-Based Methods for Gene Silencing in Mouse Oocytes.- Micro-Injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes.- Measuring Transport and Accumulation of Radiolabelled Substrates in Oocytes and Embryos.- Immunofluorescence Staining of Spindles, Chromosomes and Kinetochores in Human Oocytes.- Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes.- Chromosome Spreads with Centromere Staining in Mouse Oocytes.- Preparation of Mammalian Oocytes for Transmission Electron Microscopy.- Measuring Ca2+ Oscillations in Mammalian Eggs.- Counting Chromosomes in Intact Eggs.- Free-Hand Bisection of Mouse Oocytes and Embryos.- Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes.- Nuclear Transfer in the Mouse Oocyte.

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