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OverviewInternationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay). Full Product DetailsAuthor: Denise L. DoolanPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 2002 ed. Volume: 72 Dimensions: Width: 17.80cm , Height: 3.40cm , Length: 25.40cm Weight: 1.363kg ISBN: 9780896038233ISBN 10: 0896038238 Pages: 631 Publication Date: 28 June 2002 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Awaiting stock  The supplier is currently out of stock of this item. It will be ordered for you and placed on backorder. Once it does come back in stock, we will ship it out for you. Table of ContentsPart I. Assessing Risk of Infection and Severity of Disease Vector Incrimination and Entomological Inoculation Rates John C. Beier Epidemiological Measures of Risk of Malaria J. Kevin Baird, Michael J. Bangs, Jason D. Maguire, and Mazie J. Barcus Part II. Laboratory Models Maintenance of the Plasmodium berghei Life Cycle Robert E. Sinden, Geoff A. Butcher, and A. L. Beetsma Mouse Models for Pre-Erythrocytic-Stage Malaria Laurent Rénia, Elodie Belnoue, and Irène Landau Mouse Models for Erythrocytic-Stage Malaria Latifu A. Sanni, Luis F. Fonseca, and Jean Langhorne Nonhuman Primate Models : I. Nonhuman Primate Host-Parasite Combinations William E. Collins Nonhuman Primate Models : II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax William E. Collins Part III. Diagnosis and Typing Vector Analysis John C. Beier Genotyping of Plasmodium spp.: Nested PCR Georges Snounou Genotyping of Plasmodium falciparum: PCR-RFLP Analysis Ingrid Felger and Hans-Peter Beck Microsatellite Analysis in Plasmodium falciparum Xin-zhuan Su and Michael T. Ferdig Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR Adam A. Witney, Robert M. Anthony, and Daniel J. Carucci Quantitation of Liver-Stage Parasites by Competitive RT-PCR Kyle C. McKenna and Marcelo R. S. Briones Part IV. Molecular Biology Techniques Extraction and Purification of Plasmodium Total RNA Till Voss Extraction and Purification of Plasmodium Parasite DNA Hans-Peter Beck Southern Blotting of Parasite DNA Tobias Spielmann SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins Roland A. Cooper Nested PCR Analysis of Plasmodium Parasites Georges Snounou and BalbirSingh RFLP Analysis Hans-Peter Beck Analysis of Gene Expression by RT-PCR Peter Preiser In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites Joanne Thompson Purification of Chromosomes from Plasmodium falciparum Daniel J. Carucci, Paul Horrocks, and Malcolm J. Gardner Construction of Genomic Libraries from the DNA of Plasmodium Species Leda M. Cummings, Dharmendar Rathore, and Thomas F. McCutchan Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA Dharmendar Rathore and Thomas F. McCutchan Construction of Plasmodium falciparum l cDNA Libraries David A. Fidock, Dharmendar Rathore, and Thomas F. McCutchan Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization David A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen, and Anthony A. James Construction and Screening of YAC Libraries Cecilia P. Sanchez, Martin Preuss, and Michael Lanzer Episomal Transformation of Plasmodium berghei Chris J. Janse and Andrew P. Waters Gene Targeting in Plasmodium berghei Vandana Thathy and Robert Ménard Part V. Immunological Techniques Peptide Vaccination, Valentin Meraldi Jackeline F. Romero, and Giampietro Corradin DNA Vaccination Richard C. Hedstrom and Denise L. Doolan Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses Moriya Tsuji Assessing CD4+ Helper T-Lymphocyte Responses by Lymphoproliferation Isabella A. Quakyi and Jeffrey D. Ahlers Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice Elsa Seixas, Jean Langhorne, and Stuart Quin Cell Trafficking: Malaria Blood-Stage Parasite-Specific CD4+ T Cells After Adoptive Transfer into Mice ChakritReviewsIn this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. - Clinical Laboratory International This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters. -Molecular Biotechnology In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. - Clinical Laboratory International This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters. - Molecular Biotechnology In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. - Clinical Laboratory International This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters. -Molecular Biotechnology """In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria."" - Clinical Laboratory International ""This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters."" -Molecular Biotechnology" Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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