Overview

Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of accurate molecular structures is a pre-requisite for rational drug design, and for structure-based function studies to aid the development of effective therapeutic agents and drugs. The successful determination of the complete genome (genetic sequence) of several species (including humans) has recently directed scientific attention towards identifying the structure and function of the complete complement of proteins that make up that species; a new and rapidly growing field of study called 'structural genomics'. There are now several important and well-funded global initiatives in operation to identify all of the proteins of key model species. One of the main requirements for these initiatives is a high-throughput crystallization facility to speed-up the protein identification process. The extent to which these technologies have advanced, calls for an updated review of current crystallographic theory and practice. This practical reference book features the latest conventional and high-throughput methods, and includes contributions from a team of internationally recognized leaders and experts. It will be of relevance and use to graduate students, research scientists and professionals currently working in the field of conventional and high-throughput macromolecular crystallography.

Full Product Details

Author:   Mark R. Sanderson (Randall Division of Cell and Molecular Biophysics, King's College London, UK.) ,  Jane V. Skelly (Medway School of Science, University of Greenwich, UK.)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Dimensions:   Width: 19.40cm , Height: 2.00cm , Length: 25.30cm
Weight:   0.808kg
ISBN:  

9780198520979

ISBN 10:   0198520972
Pages:   292
Publication Date:   23 August 2007
Audience:   College/higher education ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

1: Skelly, Sohi & Batuwangala: Classical Cloning, Expression and Purification 2: Owens, Nettleship, Berrow, Stuart & Stammers: High-Throughput Cloning, Expression and Purification 3: Chayen: Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation 4: Abdel-Meguid, Jeruzalmi & Sanderson: First Analysis of Macromolecular Crystals 5: Sanderson: In-House Macromolecular Data Collection 6: Abdel-Meguid: Solving the Phase Problem Using Isomorphous Replacement 7: Delarue: Molecular Replacement Techniques for High-Throughput Structure Determination 8: Murthy: MAD Phasing 9: Weeks & Furey: Application of Direct Methods to Macromolecular Structure Solution 10: Abrahams: Phase Refinement through Density Modification 11: Morris, Perrakis & Lamzin: Getting a Macromolecular Model: Model Building, Refinement and Validation 12: Wasserman, Smith, D'Amico, Koss, Morisco, and Burley: High-Throughput Crystallographic Data Collection at Synchrotrons 13: Carson: Electron Density Fitting and Structure Validation 14: Masquida, Francois, Werner & Westhof: RNA Crystallogenesis 15: Sohi & Laponogov: Crystallography in the Study of Protein-DNA Interaction 16: Fry, Abrescia & Stuart: Virus Crystallography 17: Abdel-Meguid: Macromolecular Crystallography in Drug Design

Reviews

it is more than successful, and most, if not all, who dip into it will be rewarded by learning things they did not know, or possibly had forgotten...[..] a book that will surely find its place in every crystallographic lab whether protein or macromolecular. Lindsay Sawyer, Crystallography News

it is more than successful, and most, if not all, who dip into it will be rewarded by learning things they did not know, or possibly had forgotten...[..] a book that will surely find its place in every crystallographic lab whether protein or macromolecular. * Lindsay Sawyer, Crystallography News *

Author Information

Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins. Jane Skelly has widespread experience in the expression and crystallisation of macromolecules. Both editors lecture undergraduates in structural biology.

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