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OverviewCellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing. Full Product DetailsAuthor: Sarah L. Rowland-Jones (Institute of Molecular Medicine, Oxford) , Andrew McMichael (Institute of Molecular Medicine, Oxford) , Sarah L. Rowland-JonesPublisher: Oxford University Press Imprint: Oxford University Press Edition: 2nd Revised edition Volume: 220 Dimensions: Width: 16.10cm , Height: 2.40cm , Length: 24.10cm Weight: 0.765kg ISBN: 9780199638178ISBN 10: 0199638179 Pages: 376 Publication Date: 16 December 1999 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of ContentsPreface Plebanski: Preparation of lymphocytes and idenfication of lymphocyte subpopulations Biddulph and Gatter: Immunohistochemistry of lymphoid organs Meinl and Fickenscher: Bastin: T and B-cell hybridomas Mills: Murine T-cell culture Vyakarnam: Human CD4+ T-cell culture Rowland-Jones: Human Cytotoxic T-cell culture Carmichael: Limiting dilution analysis for quantification of antigen-specific T-cells Ogg: HLA -peptide tetrameric complexes Tan: Expansion of human T-cells for immunotheraphy Browning and Krausa: HLA typing Brown and Symons: Characterisation of lymphocyte surface markers Bangham and Furukawa: Apoptosis assays for lymphocytes Owen and McLoughlin: Thymic organ culture IndexReviews"""... the book contains extremly useful practical advice for studying lymphocytes in the laboratory..."" Parasitology Today" ... the book contains extremly useful practical advice for studying lymphocytes in the laboratory... Parasitology Today Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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