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OverviewJose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by a hands-on expert who has perfected it. Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. Full Product DetailsAuthor: Jose M. OrdovasPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 1998 ed. Volume: 110 Dimensions: Width: 15.50cm , Height: 2.00cm , Length: 23.50cm Weight: 1.360kg ISBN: 9780896034204ISBN 10: 0896034208 Pages: 284 Publication Date: 25 August 1998 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Awaiting stock  The supplier is currently out of stock of this item. It will be ordered for you and placed on backorder. Once it does come back in stock, we will ship it out for you. Table of ContentsDNA- and RNA-Based Methodolog.- PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis.- Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis.- Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method.- Generation of Transgenic Mice.- Preparation of Knockout Mice.- Protein-Based Methodology.- Fast Ultracentrifugation Methods for the Separation of Plasma Lipoproteins.- Preparative Isolation of Plasma Lipoproteins Using Fast Protein Liquid Chromatography (FPLC).- Separation of Apolipoproteins by Polyacrylamide Gel Electrophoresis.- Isolation and Purification of Serum Lipoprotein (a) Using Lectin Affinity.- Electrophoretic Separation of LDL and HDL Subclasses.- Measurement of Lipoprotein Particles.- Methodological Approaches for Assessing Lipid and Protein Oxidation and Modification in Plasma and Isolated Lipoproteins.- Separation and Quantitation of Phospholipid Classes by HPLC.- Assays of Lecithin Cholesterol Acyltransferase (LCAT).- Determination of the Mass Concentration and the Activity of the Plasma Cholesteryl Ester Transfer Protein (CETP).- In Vitro Measurement of Lipoprotein and Hepatic Lipases.- Cellular Assays for Lipoprotein Receptors.- Measurements of Proteoglycan—Lipoprotein Interaction by Gel Mobility Shift Assay.ReviewsThis text makes a good selection with admirable breadth and a high general quality of method description. -Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... -Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. -Nahrung This text makes a good selection with admirable breadth and a high general quality of method description. - Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... - Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. - Nahrung """This text makes a good selection with admirable breadth and a high general quality of method description.""-Clin Chem Lab Med ""The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists...""-Nahrung ""Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists.""-Nahrung" This text makes a good selection with admirable breadth and a high general quality of method description. -Clin Chem Lab Med The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists... -Nahrung Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists. -Nahrung Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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