Overview

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 1 focuses on shotgun and global lipidomics, analytical approaches, and lipid maps. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls. Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.

Full Product Details

Author:   Donald Armstrong
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2009 ed.
Volume:   579
Weight:   1.177kg
ISBN:  

9781617796463

ISBN 10:   1617796468
Pages:   550
Publication Date:   09 August 2012
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Shotgun and Global Lipidomics.- Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics.- Tracking the Glycerophospholipid Distribution of Docosahexaenoic Acid by Shotgun Lipidomics.- Global Analysis of Retina Lipids by Complementary Precursor Ion and Neutral Loss Mode Tandem Mass Spectrometry.- Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids.- Analytical Approaches.- Lipid Profiling Using Two-Dimensional Heteronuclear Single Quantum Coherence NMR.- Capabilities and Drawbacks of Phospholipid Analysis by MALDI-TOF Mass Spectrometry.- Lipidomics of the Red Cell in Diagnosis of Human Disorders.- HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids.- Brain Phosphoinositide Extraction, Fractionation, and Analysis by MALDI-TOF MS.- Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry.- Lipidomic Analysis of Human Meibum Using HPLC–MSn.- Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry.- A Novel Role for Nutrition in the Alteration of Functional Microdomains on the Cell Surface.- Lipidomic Analysis of Prostanoids by Liquid Chromatography–Electrospray Tandem Mass Spectrometry.- Qualitative and Quantitative Analyses of Phospholipids by LC–MS for Lipidomics.- Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting.- Dynamics of Adipose Tissue Development by 2H2O Labeling.- Analysis of Lipid Particles from Yeast.- Mammalian Fatty Acid Elongases.- Membrane Lipidomics and the Geometry of Unsaturated Fatty Acids From Biomimetic Models to Biological Consequences.- OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids.- Comprehensive Quantitative Analysis of BioactiveSphingolipids by High-Performance Liquid Chromatography–Tandem Mass Spectrometry.- The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids.- Lipid Maps.- Non-invasive Mapping of Lipids in Plant Tissue Using Magnetic Resonance Imaging.- Mapping the Lipolytic Proteome of Adipose Tissue Using Fluorescent Suicide Inhibitors.- Identifying the Spatial Distribution of Vitamin E, Pulmonary Surfactant and Membrane Lipids in Cells and Tissue by Confocal Raman Microscopy.

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