Overview

This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.

Full Product Details

Author:   Matthew C. Gage ,  Inés Pineda-Torra
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1951
Weight:   0.673kg
ISBN:  

9781493991297

ISBN 10:   1493991299
Pages:   220
Publication Date:   04 March 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS Lilia Magomedova and Carolyn L. Cummins 2. A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands Samantha A. Hutchinson and James L. Thorne 3. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-Francois Gautier, Nicolas Venteclef, and Fawaz Alzaid 4. Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages Emelie Barreby, Andre Sulen, and Myriam Aouadi 5. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity Laura Alonso-Herranz, Jesus Porcuna, and Mercedes Ricote 6. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells Ana Ramon-Vazquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo 7. Dual Crosslinking Chromatin-Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages David A. Rollins and Inez Rogatsky 8. Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics Juan Vladimir de la Rosa, Ana Ramon-Vazquez, Carlos Tabraue, and Antonio Castrillo 9. Quantifying Cellular Cholesterol Efflux Sabrina Robichaud and Mireille Ouimet 10. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection Estibaliz Glaria, Jonathan Matalonga, and Annabel F. Valledor 11. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency Matthew C. Gage 12. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution Ada Weinstock and Edward A. Fisher 13. Preparation of Frozen Liver Tissues for Integrated Omics Analysis Ning Liang, Rongrong Fan, Saioa Goni, and Eckardt Treuter 14. Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver Natalia Becares and Ines Pineda-Torra 15. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, Helene Duez, and Benoit Pourcet 16. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry Kirsty E. Waddington, Ines Pineda-Torra, and Elizabeth C. Jury

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