Overview

This volume addresses up-to-date light microscopy approaches and toolsets offered for live- or fixed-cell observations. The imaging strategies discussed in this book include confocal laser scanning and spinning disk confocal microscopy, FRET, FRAP, and laser microsurgery experiments. Chapters also describe the use of these imaging methodologies to study properties of a multitude of biomolecular targets in a broad range of model systems ranging from bacteria over tissue to whole animal imaging. Light Microscopy: Methods and Protocols puts special focus on system instrumentation parameters and sophisticated labeling and detection methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Light Microscopy: Methods and Protocols offers the novice user with straightforward strategies to address biological questions, while providing the experienced researcher with the latest applications that can be useful in routine practices. This book also serves as a useful teaching manual in practical courses on light microscopy.

Full Product Details

Author:   Yolanda Markaki ,  Hartmann Harz
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1563
Weight:   0.574kg
ISBN:  

9781493983056

ISBN 10:   1493983059
Pages:   285
Publication Date:   20 July 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface… Table of Contents… Contributing Authors… 1. Introduction to Modern Methods in Light Microscopy Part I Advanced Fluorescence Microscopy: Sample Preparation, Fluorophores and Model Systems 2. Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM) Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D. Osiewacz, and Ernst H.K. Stelzer 3. Light-Sheet Fluorescence Microscopy: Chemical Clearing and Labeling Protocols for Ultramicroscopy Nina Jährling, Klaus Becker, Saiedeh Saghafi, and Hans-Ulrich Dodt 4. Two-Photon Intravital Microscopy Animal Preparation Protocol to Study Cellular Dynamics in Pathogenesis Erinke van Grinsven, Chloe Prunier, Nienke Vrisekoop, and Laila Ritsma 5. Imaging of Brain Slices with a Genetically Encoded Voltage IndicatorPeter Quicke, Samuel J. Barnes, and Thomas Knöpfel 6. FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells Axel E. Kraft and Viacheslav O. Nikolaev 7. Imaging the Dynamics of Cell Wall Polymer Deposition in the Unicellular Model Plant, Penium margaritaceum David Domozych, Anna Lietz, Molly Patten, Emily Singer, Berke Tinaz, and Sandra C. Raimundo 8. Targeted Ablation Using Laser Nanosurgery Naga Venkata Gayathri Vegesna, Paolo Ronchi, Sevi Durdu, Stefan Terjung, and Rainer Pepperkok Part II Super and High-Resolution Optical Imaging 9. Sample Preparation and Choice of Fluorophores for Single and Dual Color Photo-Activated Localization Microscopy (PALM) with Bacterial Cells Juri N. Bach, Giacomo Giacomelli, and Marc Bramkamp 10. STED Imaging in Drosophila Brain Slices Sandra Fendl, Jesús Pujol-Marti, Joel Ryan, Alexander Borst, and Robert Kasper 11. Two-Color Total Internal Refection Fluorescence Microscopy of Exocytosis in Endocrine Cells Adam J. Trexler and Justin W. Taraska 12. Optical Coherence Microscopy Rainer A. Leitgeb Part III Quantitative and Computational Image Analysis 13. Designing Image Analysis Pipelines in Light Microscopy: A Rationale Approach Ignacio Arganda-Carreras and Philippe Andrey 14. Automated Analysis of Intracellular Dynamic Processes Yao Yao, Ihor Smal, Ilya Grigoriev, Maud Martin, Anna Akhmanova, and Erik Meijering 15. Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells José Rino, Ana C. de Jesus, and Maria Carmo-Fonseca 16. Analysis of Protein Kinetics using Fluorescence Recovery After Photobleaching (FRAP) Nickolaos-Nikiforos Giakoumakis, Maria Anna Rapsomaniki, and Zoi Lygerou 17. Fluorescence Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening Manuel Gunkel, Jan Philipp Eberle, and Holger Erfle

Reviews

“This well-illustrated book provides up-to-date light microscopy approaches and tool sets offered for live or fixed cell observations with a focus on system instrumentation parameters and sophisticated labeling and detection methods. … authors hope that this book will prove to be a robust teaching guide for light microscopy practical courses. I echo the authors’ opinion. … an excellent book which may serve as a user manual on current light microscopy approaches and toolsets for live or fixed cell observations.” (Omer Iqbal, Doody's Book Reviews, July, 2017)

This well-illustrated book provides up-to-date light microscopy approaches and tool sets offered for live or fixed cell observations with a focus on system instrumentation parameters and sophisticated labeling and detection methods. ... authors hope that this book will prove to be a robust teaching guide for light microscopy practical courses. I echo the authors' opinion. ... an excellent book which may serve as a user manual on current light microscopy approaches and toolsets for live or fixed cell observations. (Omer Iqbal, Doody's Book Reviews, July, 2017)

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