Overview

This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.  

Full Product Details

Author:   Nobuo Koizumi ,  Mathieu Picardeau
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2020
Volume:   2134
Weight:   0.772kg
ISBN:  

9781071604588

ISBN 10:   1071604589
Pages:   291
Publication Date:   07 July 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Isolation and Culture of Leptospira from Clinical and Environmental Samples.- Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database.- Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira.- Cell Enumeration of Leptospira by Flow Cytometry.- The Single-Step Method of RNA Purification Applied to Leptospira.- Purification of LPS from Leptospira.- Isolation, Purification, and Characterization of Leptophages.- Creating a Library of Random Transposon Mutants in Leptospira.- Transposon Sequencing in Leptospira interrogans.- Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9).- Leptospira spp. Toolbox for Chemotaxis Assay.- In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography.- Measurement of the Cell-Body Rotation of Leptospira.- In Vivo Imaging of Bioluminescent Leptospires.- Cell Monolayer Translocation Assay.- Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction.- Complement Resistance Assays.- Evaluation of Intracellular Trafficking in Macrophages.- Biofilm Formation and Quantification using the 96-Microtiter Plate.- Survival Tests for Leptospira spp..- Cultivation of Leptospira interrogans within Rat Peritoneal Dialysis Membrane Chambers.- Use of Golden Syrian Hamster as an Animal Model to Study Leptospirosis-Associated Immune Responses.- Evaluation of Vaccine Candidates against Leptospirosis using Golden Syrian Hamsters.- Leptospira and Leptospirosis.- Laboratory Diagnosis of Leptospirosis.

Reviews

This book is written for researchers. In my opinion, besides researchers, students, microbiologists, and others interested in knowing more about Leptospira spp. will also find this book quite useful. ... This book is an excellent resource ... . It is unique in this field by virtue of its coverage on whole genome sequencing and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and other useful techniques to study Leptospira spp. and leptospirosis. (Omer Iqbal, Doody's Book Reviews, September 18, 2020)

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