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Author:   David K. Gardner (Director of Research and Development, Director of Research and Development) ,  Michelle Lane (Senior Scientist, Senior Scientist, both at Colorado Center for Reproductive Medicine) ,  Andrew J. Watson (Associate Professor, Department of Physiology, Associate Professor, Department of Physiology, University of Western Ontario)
Publisher:   Oxford University Press Inc
Imprint:   Oxford University Press Inc
Dimensions:   Width: 27.40cm , Height: 2.30cm , Length: 21.10cm
Weight:   0.995kg
ISBN:  

9780195142266

ISBN 10:   0195142268
Pages:   432
Publication Date:   12 February 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

Preface Acknowledgements List of Contributors Abbreviations 1: Norah Spears, Evelyn E. Telfer and Alison A. Murray: Follicle development in vitro 2: Michelle Lane and David Gardner: Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery 3: David Gardner and Michelle Lane: Culture of the mammalian preimplantation embryo 4: Denny Sakkas, Michelle Lane and David Gardner: Assessment of embryo development and viability 5: Gianpiero Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, Zev Rosenwaks: Micromanipulation : ICSI 6: Joyce Harper and Alpesh Doshi: Micromanipulation : Biopsy 7: Michelle Lane, Jay Baltz and David Gardner: Analysis of intracellular ions: pH and calcium 8: David Gardner and Michelle Lane: Nutrient uptake and metabolite production and enzyme activity and regulation 9: Don Rieger: Metabolic Pathway 10: Calvin Simerly, Ricardo Moreno, João Ramalho-Santos, Laura Hewitson, and Gerald Schatten: Confocal imaging of structural molecules in mammalian gametes 11: Leeanda Wilton: Fluorescent in situ hybridization 12: W. A. King, D. H. Betts, W. Slimane and P.K. Basur: Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos 13: C. Wrenzycki, A.J. Watson, and M. Calder: Microscale RNA isolation from mammalian embryos 14: P. A. De Sousa and A. J. Watson: Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) 15: Dave Natale: Characterization of novel genes during early development by application of differential display RT-PCR 16: K. E. Latham, G. Nourjanova, K. Wigglesworth and J. J. Eppig: Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells 17: Lisa C Barcroft, D Holstead Jones-Taggart and Andrew J Watson: Antisense disruption of gene expression in the preimplantation embryo 18: M. A. Sirard and C. Robert: Gene subtraction and analysis 19: Daniel Brison Anthony D Metcalfe, Debra J Bloor, Helen R Hunter, Gerard Brady, Susan J Kimber: Analysis of apoptosis in the preimplantation embryo 20: D. P. Baluch, C. M. Pauken, and D. G. Capco: Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo 21: Susan E. Lazendorf, Catherine A. Boyd and Diane L. Wright: Human Embryonic Stem 22: Makoto Nagano: Spermatogonial Transplantion 23: Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut: Somatic Cell Nuclear Transfer 24: Tetsunori Mukaida and Magosaburo Kasai: Cryobiology; slow freezing vitrification of embryos

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