Overview

Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches considered are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed to successfully study the multifaceted world of kinesin-like proteins and to explore their many functions.

Full Product Details

Author:   Isabelle Vernos
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   164
Dimensions:   Width: 15.50cm , Height: 1.50cm , Length: 23.50cm
Weight:   0.559kg
ISBN:  

9780896037663

ISBN 10:   0896037665
Pages:   258
Publication Date:   03 December 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Purification of Kinesin from the Brain.- RT-PCR for the Identification of Developmentally Regulated Novel Members of the Kinesin-like Superfamily.- Expression Cloning with Pan Kinesin Antibodies.- Expression of Kinesin in Escherichia coli.- Plasmids for Expression of Chimeric and Truncated Kinesin Proteins.- Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System.- Assays for Kinesin Microtubule-Stimulated AT Pase Activity.- An Improved Microscope for Bead and Surface-Based Motility Assays.- Use of Photonic Force Microscopy to Study Single-Motor-Molecule Mechanics.- Assays for Microtubule-Destabilizing Kinesins.- Green Fluorescent Protein as a Tag for Molecular Motor Proteins.- In Vitro Reconstitution of Endosome Motility Along Microtubules.- Approaches to Study Interactions Between Kinesin Motors and Membranes.- Microinjection Methods for Analyzing the Functions of Kinesins in Early Embryos.- The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindle Assembly Assay in Xenopus Egg Extracts.- A Dominant Negative Approach for Functional Studies of the Kinesin II Complex.- Identification of Kinesin-Associated Proteins.- Assaying Spatial Organization of Microtubules by Kinesin Motors.- Crystallization of Kinesin.- Structural Analysis of the Microtubule–Kinesin Complex by Cryo-Electron Microscopy.

Reviews

This is a concise but comprehensive book that would be a valuable reference for any researcher who wishes to study this important cytoplasmic motor protein. It would be a valuable book to have in the laboratory as well as a useful reference book in a life sciences library. - Doody's Health Sciences Book Review Journal

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