Overview

Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression - at both the RNA and protein levels - and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.

Full Product Details

Author:   Anthony R. Howlett
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1999 ed.
Volume:   129
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   0.619kg
ISBN:  

9780896035690

ISBN 10:   0896035697
Pages:   296
Publication Date:   15 July 1999
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Working with Integrins.- Integrin-Binding Peptides Derived from Phage Display Libraries.- Production of Rat Monoclonal Antibodies Specific for Mouse Integrins.- Biochemical Analysis of Integrin-Mediated Shc Signaling.- Assays for Kinase Activity.- Immunoblotting of Integrins.- Application of Flow Cytometry in the Analysis and Sterile Sorting of Cell Populations Based on Integrin Expression.- Expression of Integrin Transcripts in Human Cancer Cells.- Functional Analysis of Integrins.- Functional Analysis of the ?2 Integrins.- Expression of Heterologous Integrin Genes.- Preparing a Polyclonal Antibody to Mouse ?1 Integrin with Function-Blocking Activity.- Integrin Gene Targeting.- Determination of Positive and Negative Regulatory Elements.- Integrin Function at the Cellular Level.- A Cell-Based Adhesion Assay for the Characterization of Integrin ?v?3 Antagonists.- Integrins in Cell Migration.- Models for Studying Cellular Invasion of Basement Membranes.- Methods for Studying Anoikis.- Use of the 10-Day-Old Chick Embryo Model for Studying Angiogenesis.- Cell Shape and Integrin Signaling Regulate the Differentiation State of Mammary Epithelial Cells.

Reviews

Integrin Protocols aims to provide a hand-book of methods for the study of integrins. Many of the essential techniques are covered, with sections ranging from the relatively straight-forward (immunoblotting) to the highly complex (phage display and knock-outs)...Over half of the sections are by major figures in the integrin field, thus giving the book considerable authority... -Cell Biology International 2000

Integrin Protocols aims to provide a hand-book of methods for the study of integrins. Many of the essential techniques are covered, with sections ranging from the relatively straight-forward (immunoblotting) to the highly complex (phage display and knock-outs)...Over half of the sections are by major figures in the integrin field, thus giving the book considerable authority... - Cell Biology International 2000

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