Overview

This volume presents a wide-ranging account of the techniques and applications of the in-vitro culture of higher plants, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are described in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test tube to soil, aids to study. The question why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

Full Product Details

Author:   R. L. M. Pierik
Publisher:   Kluwer Academic Publishers
Imprint:   Kluwer Academic Publishers
Edition:   4th Revised edition
Dimensions:   Width: 15.60cm , Height: 2.00cm , Length: 23.40cm
Weight:   0.680kg
ISBN:  

9780792352679

ISBN 10:   079235267
Pages:   348
Publication Date:   31 January 1999
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. Preface. 2. Introduction. 3. History. 4. Types of Cultures. 5. Laboratory Equipment. 6. Preparation and Composition of Nutrient Media. 7. Closure of Test Tubes and Flasks. 8. Care of Plant Material. 9. Sterilization of Plant Material. 10. Isolation, Inoculation and Subculturing. 11. Mechanization. 12. The Influence of Plant Material on Growth and Development. 13. The Influence of Physical Factors on Growth and Development. 14. The Transfer from Nutrient Medium to Soil. 15. Aids to Study. 16. Embryo Culture. 17. Germination of Orchid Seeds. 18. Vegetative Propagation of Orchids. 19. Production of Disease-Free Plants. 20. Vegetative Propagation. 21. Somaclonal Variation. 22. Test Tube Fertilization. 23. Production of Haploids. 24. Genetic Manipulation. 25. Miscellaneous Applications. 26. In Vitro Cloning of Plants in the Netherlands. 27. Index. 28. Literature Cited.

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