Overview

This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.

Full Product Details

Author:   Omar Bagasra (Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania) ,  John Hansen (MJ Research, Inc., Watertown, Massachusetts)
Publisher:   John Wiley & Sons Inc
Imprint:   Wiley-Liss Inc.,U.S.
Dimensions:   Width: 16.00cm , Height: 1.50cm , Length: 23.00cm
Weight:   0.272kg
ISBN:  

9780471159469

ISBN 10:   0471159468
Pages:   160
Publication Date:   18 July 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Overview; Review of the PCR Technique; Preliminary Solution-Based Reactions; Preparation of Glass Slides and Tissues; In Situ PCR: DNA and RNA Targets; Special Applications of In Situ Amplification; Hybridization Reactions; Validation and Controls; Materials and Methods; Select Biography; Appendices; Index; About the Authors.

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Author Information

Omar Bagasra is with Claflin University in Orangeburg, South Carolina, USA.

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