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Author:   Boye Schnack Nielsen ,  Julia Jones
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   5th ed. 2020
Volume:   2148
Weight:   0.773kg
ISBN:  

9781071606254

ISBN 10:   1071606255
Pages:   392
Publication Date:   12 May 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Guidelines for the Optimization and Validation of In Situ Hybridization.- Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization.- Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories.- Fluorescent In Situ Hybridization using Oligonucleotide-Based Probes.- Visualizing Genome Reorganization using 3D DNA FISH.- MicroRNA In Situ Hybridization in Paraffin Embedded Cultured Cells.- Multiplexed Detection and Analysis of Low Abundant Abundance Long Non-Coding RNA using RNAscopeTM in Cultured Cells.- Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0).- Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0).- Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution within Whole-Mount VertebrateEmbryos: qHCR and dHCR imaging (v3.0).- Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues.- RNAscopeTM Multiplex Detection in Zebrafish.- Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues using RNAscopeTM.- Automated ISH for Validated Histological Mapping of Lowly Expressed Genes.- Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.- Automated Co-In Situ Hybridization and Immunofluorescence using Archival Tumor Tissue.- Automated 5-Color Multiplex Co-Detection of MicroRNA and Protein Expression in Fixed Tissue Specimens.- Mixed Multiplex Staining: Automated RNAscopeTM and OPALTM for Multiple Targets.- Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscopeTM In Situ Hybridization and Immunofluorescence Protocol.- In SituSequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections.- GeoMx™ RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue.- In Situ Point Mutation Detection in FFPE Colorectal Cancers using the BaseScope Assay.- Using In Situ Padlock Probe Technology to Detect mRNA Splice Variants in Tumor Cells.- Automated One-Double-Z Pair BaseScope™ for CircRNA In Situ Hybridization.

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