In Situ Hybridization: Principles and Practice

Author:   Julia M. Polak (, Department of Histochemistry, Royal Postgraduate Medical School, London) ,  James O'D. McGee (, University of Oxford Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Oxford) ,  Mushirul Hasan
Publisher:   Oxford University Press
Edition:   2nd Revised edition
ISBN:  

9780198548805


Pages:   224
Publication Date:   28 January 1999
Format:   Hardback
Availability:   To order   Availability explained
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In Situ Hybridization: Principles and Practice


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Overview

This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take into account the many advances in the last seven years.

Full Product Details

Author:   Julia M. Polak (, Department of Histochemistry, Royal Postgraduate Medical School, London) ,  James O'D. McGee (, University of Oxford Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital, Oxford) ,  Mushirul Hasan
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Dimensions:   Width: 15.90cm , Height: 1.70cm , Length: 24.00cm
Weight:   0.507kg
ISBN:  

9780198548805


ISBN 10:   019854880
Pages:   224
Publication Date:   28 January 1999
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   To order   Availability explained
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

1: Principles of in situ hybridization 2: The design and generation of probes for in situ hybridization 3: The preparation of non-radioisotopic hybridization probes 4: Principles and applications of complementary RNA probes 5: Quantification of in situ hybridization 6: Quantification of grains in in situ hybridization 7: Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization 8: Single and simultaneous nucleic acid detection in clinical samples 9: Combined in situ hybridization and immunocytochemistry: investigation of viral pathogenesis at the light and electron microscope levels 10: Super sensitive in situ DNA and RNA localisation

Reviews

Anyone contemplating using in situ hybridisation in their laboratory will need a thorough grounding in the background and principles of the methods. This book excels in its background presentation, with clear explanations and good referencing...There are very fewbooks dealing with the practicalities of in situ hybridisation. This one makes a major contribution to the subject and therefore is invaluable as a reference for many scientists and clinicians Dr Deborah K Dun-Waters, The Bulletin of the Royal College of Pathologists, No. 107 June 1999


Anyone contemplating using in situ hybridisation in their laboratory will need a thorough grounding in the background and principles of the methods. This book excels in its background presentation, with clear explanations and good referencing.....There are very fewbooks dealing with the practicalities of in situ hybridisation. This one makes a major contribution to the subject and therefore is invaluable as a reference for many scientists and clinicians Dr Deborah K Dun-Waters, The Bulletin of the Royal College of Pathologists, No. 107 June 1999


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