Overview

"The detection of specific nucleic acid sequences by in situ hybridization has many important applications in modern biology. In situ detection of viral DNA sequences is enabling the diagnosis of human diseases, the detection of specific sequences on chromosomes is crucial for genome analysis, and in fields such as developmental biology and neurobiology, the investigation of gene expression by in situ hybridization to mRNA has become a key technique. ""In Situ Hybridization - A Practical Approach"" is a comprehensive practical guide to the uses of this major technique. Step-by-step protocols are presented for the preparation of cloned DNA, RNA and oligonucleotide probes, with both radioactive and non-radioactive labels, and their hybridization to mRNA, viral DNA, and chromosomal DNA. Methods are provided for the analysis of cells, tissue sections, and whole embryos. In addition, techniques are presented for carrying out in situ hybridization in combination with the immunochemical detection of protein, and for using it at the electron microscopic level. This book is aimed at any researcher wishing to exploit the many widesprad applications of this important technique."

Full Product Details

Author:   D. G. Wilkinson
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   No. 109
Dimensions:   Width: 15.60cm , Height: 1.60cm , Length: 23.40cm
Weight:   0.496kg
ISBN:  

9780199633289

ISBN 10:   0199633282
Pages:   180
Publication Date:   01 October 1992
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Replaced By:   9780199636594
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions