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OverviewThe popularly grown wheat is allohexaploid (2n=6X=42), consisting of three closely related sub-genomes, A, B and D. The polyploid inheritance, large genome size (~17Gb) and significant amount (~95%) of repetitive DNA render wheat recalcitrant to forward and reverse genetics approaches. In order to better understand the composition and organization of wheat genome, techniques of Cot fractionation and methylation-filtration were used. For this purpose, three different genomic DNA libraries (one each from methylation-filtered, high Cot and reassociated DNA fractions) were prepared, and random clones from each of the three libraries were sequenced. The resulting sequences were used for studying structural (protein coding and non-coding RNA genes, transposable elements, etc) and compositional (GC content) differences among the three fractions. Availability of >1 million wheat ESTs in the public domain allowed development of gene-derived markers (SSRs and SNPs). These markers were used for studying genetic diversity among the elite wheat genotypes, and studying transferability to wild wheat relatives, which can serve as pool of useful alleles for future wheat improvement. Full Product DetailsAuthor: Sachin RustgiPublisher: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Imprint: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Dimensions: Width: 22.90cm , Height: 0.80cm , Length: 15.20cm Weight: 0.233kg ISBN: 9783639332278ISBN 10: 363933227 Pages: 152 Publication Date: 10 February 2011 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: Not yet available  This item is yet to be released. You can pre-order this item and we will dispatch it to you upon its release. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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