Overview

This much anticipated third edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques . It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

Full Product Details

Author:   Robert Burns
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   3rd ed. 2005
Volume:   295
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   0.642kg
ISBN:  

9781588292742

ISBN 10:   1588292746
Pages:   318
Publication Date:   13 December 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Immunization Strategies for Antibody Production.- Synthetic Peptides as Antigens for Antibody Production.- Production of Polyclonal Antibodies in Rabbits.- Making Hybridomas.- Growing Hybridomas.- Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments.- Purification of Antibodies and Preparation of Antibody Fragments.- Conjugation of Antibodies to Alkaline Phosphatase.- Conjugation of Antibodies to Horseradish Peroxidase.- Conjugation of Antibodies to Fluorescein or Rhodamine.- Conjugation of Antibodies to Biotin.- Preparation of Colloidal Gold Probes.- Immunogold Probes in Light Microscopy.- Immunoelectron Microscope Techniques in Plant Virus Diagnosis.- Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay.- Competitive Enzyme-Linked Immunosorbent Assay.- Immunoblotting Techniques.- Epitope Mapping.- Generation and Testing of Immunoaffinity Columns.- Immunocapture-Polymerase Chain Reaction.- Magnetic Cell Sorting.- Cell Enrichment and Immunochemical Staining.

Reviews

Reviews of Previous editions: a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive will be useful to both those who already have some experience in this area and to those who wish to embark on new techniques. - Microbiology Today An excellent and well arranged compilation of a range of antibody techniques...An excellent laboratory manual. - Immunoassay

Reviews of Previous editions: ...a treasury of detailed information, both within the protocols and in the Notes, which in some chapters are extensive...will be useful to both those who already have some experience in this area and...to those who wish to embark on new techniques. -Microbiology Today An excellent and well arranged compilation of a range of antibody techniques...An excellent laboratory manual.-Immunoassay

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