Overview

This practical volume is designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are also described. The text goes on to explain solid-phase reagents, standards and immunogens, with procedures for their preparation. Most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

Full Product Details

Author:   J.R.G. Gosling
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   Revised ed.
Volume:   No.228
Dimensions:   Width: 18.90cm , Height: 2.30cm , Length: 24.60cm
Weight:   0.752kg
ISBN:  

9780199637119

ISBN 10:   0199637113
Pages:   324
Publication Date:   01 September 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

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