Overview

This volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Elaine Bignell
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2260
Weight:   0.493kg
ISBN:  

9781071611845

ISBN 10:   1071611844
Pages:   242
Publication Date:   07 January 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans.- Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification.- CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia.- Analysis of Epithelial Cell Responses to Microbial Pathogens.- Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry.- Fungal Bioreporters to Monitor Outcomes of Blastomyces – Host Cell Interactions.- Fungal Bioreporters to Monitor Outcomes of Aspergillus – Host Cell Interactions.- Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays.- Experimental Evolution of Candida by Serial Passaging in Host Cells.- Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells.- Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras.- Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole.- Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections.- A Murine Model for Chronic A. fumigatus Airway Infections.- Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge. 

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions