Overview

This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.

Full Product Details

Author:   Guido Poli ,  Elisa Vicenzi ,  Fabio Romerio
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2407
Weight:   0.883kg
ISBN:  

9781071618738

ISBN 10:   1071618733
Pages:   451
Publication Date:   07 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Cell lines with Minimal but Inducible Proviral Expression 1. Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency Anthony RODARI, Guido Poli, and Carine VAN LINT   2. U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency Guido Poli   Part II: In vitro and ex vivo primary cell models of minimal but inducible proviral expression 3. An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency in primary human CD4+ T cells Rui Li and Fabio Romerio   4. The cultured TCM model of HIV latency Alberto Bosque   5. A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) MethodMeenakshi Shukla, Fredrick Kizito, Uri Mbonye, Kien Nguyen, Curtis Dobrowolski, and Jonathan Karn   6. TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model Sydney Bergstresser and Deanna A. Kulpa   7. Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification Amélie Cattin, Augustine Fert, Delphine Planas, and Petronela Ancuta   8. Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF) Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, and Carlo Federico Perno   9. Human monocyte-derived macrophages (MDM) - Model 2 Francesca Graziano, Elisa Vicenzi, and Guido Poli   10. Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100 Amelie Bauer   11. Hematopoietic stem and progenitor cells (HSPCs) Valeri H. Terry, Gretchen E. Zimmerman, Maria C. Virgilio, Mark M. Painter, Dale Bixby, and Kathleen L. Collins   Part III: In vitro and ex vivo tissue-derived models of reversible proviral latency 12. HIV reservoirs: Modeling, Quantification and Approaches to a Cure Louise A. Ouattara, Nikolas C. Vann, and Gustavo F. Doncel   13. More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication Jean-Pierre Routy, Franck P. Dupuy, John Lin, and Stéphane Isnard   Part IV: Animal Models 14. Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells Fernando Real, Yonatan Ganor, and Morgane Bomsel   15. Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond Nabila Jabrane-Ferrat and Hicham El Costa   16. HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure Amir Dashti, Vidisha Singh, and Ann Chahroudi   17. Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research Triana Rivera-Megias, Nhut M Le, and Alonso Heredia   18. Inducing long-term HIV-1 latency in the TKO-BLT mouse model Yunyun Di and Kerry J. Lavender   Part V: Methods for detection and analysis of the reservoir 19. In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue Kathleen Busman-Sahay, Michael D. Nekorchuk, Carly Elizabeth Starke, Chi Ngai Chan, and Jacob D. Estes   20. Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH Mathieu Dubé and Daniel E. Kaufmann   21. Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays Elizabeth R. Wonderlich, Monica D. Reece, and Deanna A. Kulpa   22. Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells Abreu CM, Veenhuis RT, Shirk EN, Queen SE, Bullock BT, Mankowski JL, Gama L and Clements JE   23. Near-full-length single-genome HIV-1 DNA sequencing Guinevere Q. Lee and Mathias Lichterfeld   24. TILDA: Tat/rev Induced Limiting Dilution Assay Cynthia Lungu and Francesco A. Procopio   Part VI: Cure and long-term remission strategies 25. Latency Reversal and Clearance of Persistent HIV Infection David M. Margolis   26. Cure and long-term remission strategies Luisa Mori and Susana T. Valente   27. Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy Jonathan Herskovitz, Mahmudul Hasan, Milankumar Patel, Bhavesh D Kevadiya,and Howard E. Gendelman

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