Overview

AIDS is one of the most pressing medical emergencies of our era. Despite arguments to the contrary, the majority of AIDS researchers have agreed that there is a causal link between HIV infections and AIDS. However, it is now 12 years since the discovery of HIV and we are still a long way from understanding and controlling AIDS. HIV 1 and HIV 2 are guides to studying HIV in the laboratory. HIV 1 describes studies of the virus and infected cells and covers techniques for handling HIV safely, for studying viral entry, replication, and neutralization, and basic immunological techniques. HIV 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described, plus assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery.HIV research is highly multidisciplinary so a complete compendium of all related methods would have been unreadable. Instead, theses two volumes focus on the specialized techniques which are unique to studies of HIV. The books have been designed so that a novice entering HIV research can find clear instructions about how to perform standard experiments, such as assays for reverse transcriptase or antibodies to HIV. In addition the books include many novel methods which are described in detail for the first time.

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The two volumes are of exceptional value for laboratory workers studying the many different aspects of HIV and HIV disease. At an affordable price, these two volumes provide a wealth of information on standard techniques and on more sophisticated approaches for the study of the interaction between cellular and viral proteins. It is a comprehensive manual which serves as a solid basis for all investigators who are exploring this complex virus and the complex pathogenesis of HIV disease. Prferably in the form of multiple copies, it deserves a place on the bookshelf of any laboratory working with HIV. * M.A. Koch, Zentralblatt fur Bakteriologie 1996 * HIV: a Practical Approach is the latest two-volume offering from the Practical Approach series and provides the first comprehensive methodology for the HIV field. As such, it can be recommended strongly to all active bench scientists in the field as well as to desk-bound research directors who wish to keep in touch ... well worth the effort. * James C. Neil, University of Glasgow Veterinary School, Molecular Medicine Today, October 1996 *

HIV: a Practical Approach is the latest two-volume offering from the Practical Approach series and provides the first comprehensive methodology for the HIV field. As such, it can be recommended strongly to all active bench scientists in the field as well as to desk-bound research directors who wish to keep in touch ... well worth the effort. James C. Neil, University of Glasgow Veterinary School, Molecular Medicine Today, October 1996 The two volumes are of exceptional value for laboratory workers studying the many different aspects of HIV and HIV disease. At an affordable price, these two volumes provide a wealth of information on standard techniques and on more sophisticated approaches for the study of the interaction between cellular and viral proteins. It is a comprehensive manual which serves as a solid basis for all investigators who are exploring this complex virus and the complex pathogenesis of HIV disease. Prferably in the form of multiple copies, it deserves a place on the bookshelf of any laboratory working with HIV. M.A. Koch, Zentralblatt fur Bakteriologie 1996

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