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OverviewThe molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins. Full Product DetailsAuthor: Paul Millner (School of Biochemistry and Molecular Biology, University of Leeds)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 204 Dimensions: Width: 16.00cm , Height: 2.10cm , Length: 24.10cm Weight: 0.705kg ISBN: 9780199636495ISBN 10: 0199636494 Pages: 333 Publication Date: 29 April 1999 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of ContentsSeparation by charge, size and hydrophobicity Practice and benefits of microcolumn HPLC Detection devices Capillary electrophoresis of peptides and proteins Lectins as affinity probes Nucleotide-and dye-ligand chromatography Synthetic peptides as affinity ligands Drugs and inhibitors as affinity ligands Immunoaffinity chromatography Strategies and methods for purification of Ca2+ -binding proteins Purification of DNA-binding proteinsReviewsLaboratory workers will find this a useful guide to the development of effective purification protocols Aslib Book Guide Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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