Overview

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

Full Product Details

Author:   Isabelle Mus-Veteau
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2016
Volume:   1432
Dimensions:   Width: 17.80cm , Height: 1.80cm , Length: 25.40cm
Weight:   6.322kg
ISBN:  

9781493981021

ISBN 10:   1493981021
Pages:   322
Publication Date:   30 May 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies.- Membrane Protein Production in the Yeast, S. cerevisiae.- Membrane Proteins Production in Escherichia coli: Protocols and Rules.- Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach.- Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins.- Membrane Protein Production in Lactococcus lactis for Functional Studies.- Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells.- Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6.- Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA.- Expression of Eukaryotic Membrane Proteins in Pichia pastoris.- Integral Membrane Protein Expression in Saccharomyces cerevisiae.- High-Throughput Baculovirus Expression System for Membrane Protein Production.- Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies.- Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies.- Membrane Protein Solubilization and Composition of Protein Detergent Complexes.- Detergent-Free Membrane Protein Purification.- Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.- Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization.- Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter.

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