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OverviewFull Product DetailsAuthor: Lu-Yun Lian (The University of Liverpool) , Gordon Roberts (University of Leicester, UK)Publisher: John Wiley & Sons Inc Imprint: John Wiley & Sons Inc Dimensions: Width: 17.60cm , Height: 2.50cm , Length: 24.70cm Weight: 0.807kg ISBN: 9780470721933ISBN 10: 0470721936 Pages: 368 Publication Date: 22 July 2011 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Out of stock  The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available. Table of ContentsList of Contributors xiii Introduction 1 Lu-Yun Lian and Gordon Roberts References 4 1 Sample Preparation, Data Collection and Processing 5 Frederick W. Muskett 1.1 Introduction 5 1.2 Sample Preparation 5 1.3 Data Collection 11 1.4 Data Processing 17 2 Isotope Labelling 23 Mitsuhiro Takeda and Masatsune Kainosho 2.1 Introduction 23 2.2 Production Methods for Isotopically Labelled Proteins 24 2.3 Uniform Isotope Labelling of Proteins 29 2.4 Selective Isotope Labelling of Proteins 32 2.5 Segmental Labelling 37 2.6 SAIL Methods 38 2.7 Concluding Remarks 45 3 Resonance Assignments 55 Lu-Yun Lian and Igor L. Barsukov 3.1 Introduction 55 3.2 Resonance Assignment of Unlabelled Proteins 56 3.3 15N-Edited Experiments 60 3.4 Triple Resonance 62 3.5 Side-Chain Assignments 77 4 Measurement of Structural Restraints 83 Geerten W. Vuister, Nico Tjandra, Yang Shen, Alex Grishaev and Stephan Grzesiek 4.1 Introduction 83 4.2 NOE-Based Distance Restraints 84 4.3 Dihedral Restraints Derived from J-Couplings 96 4.4 Hydrogen Bond Restraints 103 4.5 Orientational Restraints 107 4.6 Chemical Shift Structural Restraints 129 4.7 Solution Scattering Restraints 137 5 Calculation of Structures from NMR Restraints 159 Peter Guntert 5.1 Introduction 159 5.2 Historical Development 161 5.3 Structure Calculation Algorithms 164 5.4 Automated NOE Assignment 173 5.5 Nonclassical Approaches 178 5.6 Fully Automated Structure Analysis 181 6 Paramagnetic Tools in Protein NMR 193 Peter H.J. Keizers and Marcellus Ubbink 6.1 Introduction 193 6.2 Types of Restraints 194 6.3 What Metals to Use? 200 6.4 Paramagnetic Probes 203 6.5 Examples 209 6.6 Conclusions and Perspective 212 7 Structural and Dynamic Information on Ligand Binding 221 Gordon Roberts 7.1 Introduction 221 7.2 Fundamentals of Exchange Effects on NMR Spectra 222 7.3 Measurement of Equilibrium and Rate Constants 229 7.4 Detecting Binding – NMR Screening 238 7.5 Mechanistic Information 241 7.6 Structural Information 246 8 Macromolecular Complexes 269 Paul C. Driscoll 8.1 Introduction 269 8.2 Spectral Simplification through Differential Isotope Labelling 270 8.3 Basic NMR Characterisation of Complexes 273 8.4 3D Structure Determination of Macromolecular Protein–Ligand Complexes 277 8.5 Literature Examples 297 9 Studying Partially Folded and Intrinsically Disordered Proteins Using NMR Residual Dipolar Couplings 319 Malene Ringkjøbing Jensen, Valery Ozenne, Loic Salmon, Gabrielle Nodet, Phineus Markwick, Pau Bernado´ and Martin Blackledge 9.1 Introduction 319 9.2 Ensemble Descriptions of Unfolded Proteins 320 9.3 Experimental Techniques for the Characterisation of IDPs 320 9.4 NMR Spectroscopy of Intrinsically Disordered Proteins 321 9.5 Residual Dipolar Couplings 323 9.6 Conclusions 340 References 340 Index 347Reviews?This is a must for anyone interested in using solution NMR to study proteins. Summing Up: Highly recommended. Graduate students, researchers/faculty, and professionals/practitioners.? (Choice, 1 April 2012) <colgroup span= 1 ><col span= 1 width= 221 >“This is a must for anyone interested in using solution NMR to study proteins. Summing Up: Highly recommended. Graduate students, researchers/faculty, and professionals/practitioners. (Choice, 1 April 2012) <colgroup span= 1 ><col span= 1 width= 221 > This is a must for anyone interested in using solution NMR to study proteins. Summing Up: Highly recommended. Graduate students, researchers/faculty, and professionals/practitioners. (Choice, 1 April 2012) Author InformationProfessor Gordon Roberts is Head of the School of Biological Sciences, University of Leicester. Dr Christina Redfield is Reader, Oxford Centre for Molecular Sciences, University of Oxford. Tab Content 6Author Website:Countries AvailableAll regions |
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