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OverviewGuide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology. The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text: Lentivirus transgenesis o Germline modification using mouse stem cells Electroporation Applications of transposons in mouse genetics Functional genomics using transposon systems The use of DNA transposons in detecting cancer genes in mice Recombination, conditional mutagenesis and induction of tamoxifen Genetic fate mapping using recombinases Genetic screens mouse ES cells Gene trap mutagenesis Mouse mutagenesis Self- renewal and pluripotency Transgenic RNAi applications Gene knockdowns Tetracycline-controlled transcription Gene expression profiling of mouse embryos The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice. Full Product DetailsAuthor: Paul Wassarman (Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA) , Philippe M. Soriano (Professor of Cell, Developmental and Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA)Publisher: Elsevier Science Publishing Co Inc Imprint: Academic Press Inc Edition: 2nd ed. Dimensions: Width: 15.20cm , Height: 2.80cm , Length: 22.90cm Weight: 1.000kg ISBN: 9780123848826ISBN 10: 0123848822 Pages: 628 Publication Date: 24 September 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsSection I. Transgenesis 1. Lentivirus transgenesis 2. Germline modification using mouse spermatogonial stem cells 3. Embryonic in vivo electroporation in the mouse Section II. Transposons 4. Current applications of transposons in mouse genetics 5. Functional genomics in the mouse using the Sleeping Beauty transposon system 6. The use of DNA transposons for cancer gene discovery in mice Section III. Recombinases 7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2 8. A recombineering pipeline to make conditional targeting constructs 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria 10.Genetic fate mapping using site-specific recombinases 11. Mapping cell fate and function using recombinase-based intersectional strategies Section IV. Mutagenesis 12. Genome-wide forward genetic screens in mouse ES cells 13. Gene trap mutagenesis in the mouse 14. A wider context for gene trap mutagenesis 15. Mouse mutagenesis with the chemical supermutagen ENU 16. Phenotype-driven mouse ENU mutagenesis screens 17. Using ENU mutagenesis for phenotype-driven analysis of the mouse Section V. Gene Knockdowns 18. Exploration of self-renewal and pluripotency in ES cells using RNAi 19. Transgenic RNAi applications in the mouse 20. Gene knockdown in the mouse through RNAi 21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice 22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription Section VI. Gene Expression Profiling 23. Gene expression profiling of mouse oocytes and preimplantation embryos 24. Interrogating the transcriptome of oocytes and preimplantation embryos 25. Gene expression profiling of mouse embryos with microarraysReviewsAuthor InformationPhilippe Soriano,is Professor of Cell, Developmental & Regenerative Biology and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA Tab Content 6Author Website:Countries AvailableAll regions |
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