Overview

Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for the analysis of mucins.

Full Product Details

Author:   Anthony P. Corfield
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   125
Dimensions:   Width: 15.50cm , Height: 2.80cm , Length: 23.50cm
Weight:   2.010kg
ISBN:  

9780896037205

ISBN 10:   0896037207
Pages:   506
Publication Date:   01 March 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Purification of Mucin.- Isolation of Large Gel-Forming Mucins.- Preparation of Membrane Mucin.- Detection and Ouantitation of Mucin.- Histologically Based Methods for Detection of Mucin.- Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods.- The Gastrointestinal Adherent Mucous Gel Barrier.- Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling.- Separation, Identification, and Physical Characterization of Mucins.- Separation and Identification of Mucins and Their Glycoforms.- Heterogeneity and Size Distribution of Gel-Forming Mucins.- Rheology of Mucin.- Rheology of Mucin.- Mucin Peptide Analysis.- Amino Acid Analysis of Mucins.- Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation.- Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies.- Mucin Domains to Explore Disulfide-Dependent Dimer Formation.- Carbohydrate Structural Analysis of Mucins.- Monosaccharide Composition of Mucins.- O-Linked Oligosaccharide Chain Release and Fractionation.- Structural Analysis of Mucin-Type O-Linked Oligosaccharides.- Measurement of Sulfate in Mucins.- Mucin Biosynthesis.- Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study.- Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin.- Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis.- Mucin Precursors Identification and Analysis of Their Intracellular Processing.- Inhibition of Mucin Glycosylation.- O-Linked Chain Glycosyltransferases.- Mucin Gene Detection.- Mucin cDNA Cloning.- Northern Blot Analysis of Large mRNAs.- Southern Blot Analysis of Large DNA Fragments.- In Situ Hybridization Techniques for Localizing Mucin mRNA.- Detection of Mucin Gene Polymorphism.- Preparation of Antimucin Antibodies.- Polyclonal and Monoclonal Techniques.- Monoclonal Antibodies to Mucin VNTR Peptides.- Enzymatic Degradation of Mucins.- Mucinase Activity.- Proteinase Activity.- Glycosidase Activity.- Assays for Bacterial Mucin-Desulfating Sulfatases.- Mucin-Bacterial Interactions.- Mucin-Bacterial Binding Assays.- Growth of Mucin Degrading Bacteria in Biofilms.- Cellular And Humoral Responses To Mucins.- Generation of MUC1 CytotoxicT-Cells in Mice and Epitope Mapping.- Analysis of the Frequency of MHC-Unrestricted MUC1 -Specific CytotoxicT-Cells in Peripheral Blood by Limiting Dilution Assay.- Expression of MUC1 in Insect Cells Using Recombinant Baculovirus.- Mucin Antigen Presentation Using Dendritic Cells.- Detection of Humoral Immune Responses to Mucins.

Reviews

There is sufficient detail to follow easily and unlike many research papers, the minute details which make for success of failure, all appear to included. -Microbiology Today

There is sufficient detail to follow easily and unlike many research papers, the minute details which make for success of failure, all appear to included. - Microbiology Today

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