Overview

This detailed volume presents a variety of specialized analytical methods on a wide range of glycolipids. From sphingoglycolipids to lipid A, an immune system stimulant present in lipopolysaccharide, the book delves into how glycolipid structure on the surface of the cell membrane, which characterizes each organism, is crucial for the coexistence or repulsion between organisms. In addition, glycolipids play an important role in infections in animals and other organisms by targeting host cell membranes, if enveloped viruses are considered as living organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Glycolipids: Methods and Protocols serves as an ideal guide to themost advanced glycolipid research in an effort to help researchers make their research more fruitful.

Full Product Details

Author:   Kazuya Kabayama ,  Jin-ichi Inokuchi
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2613
Weight:   0.794kg
ISBN:  

9781071629093

ISBN 10:   1071629093
Pages:   302
Publication Date:   02 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Immunomodulatory Functions of α-GalCer and a Derivative, α-Carba-GalCer.- Chemical Synthesis and Molecular Interaction Analysis of a-Galactosyl Ceramide Derivatives as CD1d Ligands.- Immunomodulatory Functions of Glycolipids from Pathogens.- Chemical Synthesis and Immunomodulatory Functions of Bacterial Lipid As.- Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines.- Chemical Synthesis of Phosphatidylglucoside.- Sialidase-Resistant Ganglioside GM3 Analogues: Evaluation of Biological Activity.- Chemical Synthesis of Gangliosides.- Sialyltransferase Activity Assay for Ganglioside GM3 Synthase.- Construction of Sphingolipid Remodeled Cells by Genome Editing.- Mass Spectrometry of Neutral Glycosphingolipids.- Structural Analyses of the Glycolipids in Lipid Rafts.- Extraction, Purification, and Chemical Degradation of LPS from Gut Microbiota Strains.- NMR Analysis of Mammalian Glycolipids.- Distribution of Glycolipids in the Plasma Membrane Monitored by Specific Probes inCombination with Sodium Dodecyl Sulfate-Digested Freeze-Fracture Replica Labeling (SDS-FRL).- Biochemical and Microscopic Analyses for Sphingolipids and Its Related Molecules in Phagosomes.- Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes.- Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi.- Molecular Dynamics of Glycolipids in Liposomes.- Inhibitors of Glucosylceramide Synthase.- Comprehensive Glycan Analysis of Sphingolipids in Human Serum/Plasma.

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