Overview

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect - full-text online of volumes 1 onwards. This volume features methods for the study of globin and other nitric oxide-reactive proteins.

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Author:   Robert K. Poole (West Riding Professor of Microbiology, Department of Molecular Biology and Biotechnology, University of Sheffield, UK) ,  Robert K. Poole (University of Sheffield, UK) ,  Robert K. Poole (University of Sheffield, UK) ,  Robert K. Poole (University of Sheffield, UK)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   436
Dimensions:   Width: 15.20cm , Height: 3.10cm , Length: 22.90cm
Weight:   1.000kg
ISBN:  

9780123742773

ISBN 10:   0123742773
Pages:   672
Publication Date:   20 March 2008
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Section I. Nitric Oxide: Chemical and Analytical Methods Chapter 1 The Chemistry of Nitric Oxide and Related Species Martin H. Hughes Chapter 2 Delivery of nitric oxide for analysis of the function of cytochrome c'. Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir Chapter 3 The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems Rubina G. Aga and Martin N. Hughes Chapter 4 The Chemistry of Peroxynitrite - Implications for Biological Activity Sara Goldstein and Gabor Merenyi Chapter 5 Nitric Oxide (NO) Selective Electrodes Ian R. Davies, Xueji Zhang Chapter 6 NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries Chapter 7 Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples Jay R. Laver, Tania M. Stevanin, Robert C. Read Section II. Bacterial and Archaeal Hemoglobins Chapter 8 Interactions of nitric oxide with hemoglobin: from microbes to man Michael Angelo, Alfred Hausladen and Jonathan S. Stamler Chapter 9 Expression and purification of E. coli Hmp Robert K. Poole Chapter 10 Structural studies on flavohemoglobins Andrea Ilari and Alberto Boffi Chapter 11 Flavohemoglobin of Staphylococcus aureus Ligia S. Nobre, Vera L. Goncalves and Ligia M. Saraiva Chapter 12 Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins Paul R. Gardner Chapter 13 Globin Interactions with Lipids and Membranes Antonio Di Giulio and Alessandra Bonamore Chapter 14 Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey Chapter 15 Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes Xiao-Xing WEI, Guo-Qiang CHEN Chapter 16 Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuni James L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. Poole Chapter 17 Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins Alessandra Pesce, Mario Milani, Marco Nardini and Martino Bolognesi Chapter 18 Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins Paolo Ascenzi and Paolo Visca Section III. Other Hemoglobins Chapter 19 Expression, purification and crystallisation of neuro-and cytoglobin Sylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc Monees Chapter 20 Measurement of distal histidine coordination equilibrium and kinetics in hexacoordinate hemoglobins. Benoit J. Smagghe, Puspita Halder, and Mark S. Hargrove Chapter 21 Purification of Class 1 Plant Hemoglobins and Examination of their Functional Properties Abir U. Igamberdiev and Robert D. Hill Chapter 22 Use of in silico (computer) methods to predict and analyze the tertiary structure of plant hemoglobins Sabarinathan Kuttalingam Gopalasubramaniam, Veronica Garrocho-Villegas, Genoveva Bustos Rivera, Nina Pastor and Raul Arredondo-Peter Chapter 23 A Self-induction Method to Produce High Quantities of Recombinant Functional Flavo-Leghemoglobin Reductase. Estibaliz Urarte, Inigo Auzmendi, Selene Rol, Idoia Ariz, Pedro Aparicio-Tejo, Raul Arredondo-Peter and Jose F. Moran Chapter 24 Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins Alessandro Vergara, Luigi Vitagliano, Cinzia Verde, Guido di Prisco, Lelio Mazzarella Chapter 25 Dinitrosyl Iron Complexes Bound with Haemoglobin as Markers of Oxidative Stress Konstantin B. Shumaev, Olga V. Kosmachevskaya, Alexandr A. Timoshin, Anatoly F. Vanin and Alexey F. Topunov Chapter 26 Linked analysis of large cooperative, allosteric systems: the case of the giant HBL hemoglobins Nadja Hellmann, Roy E. Weberb and Heinz Decker Chapter 27 Mass Mapping of Large Globin Complexes by Scanning Transmission Electron Microscopy Joseph S. Wall, Martha N. Simon, Beth Y. Lin, Serge N. Vinogradov Chapter 28 Mini-Hemoglobins from Nemertean Worms Thomas L. Vandergon and Austen F. Riggs Chapter 29 Comparative and Evolutionary Genomics of Globin Genes in Fish Enrico Negrisolo, Luca Bargelloni, Tomaso Patarnello, Catherine Ozouf-Costaz, Eva Pisano, Guido di Prisco, Cinzia Verde Chapter 30 Inferring Evolution of Fish Proteins: The Globin Case Study Agnes Dettai, Guideo de Prisco, Guillaume Lecointre, Elio Parisi, Cinzia Verde Chapter 31 Tracing Globin Phylogeny Using PSI-BLAST Searches Based on Groups of Sequences Serge N. Vinogradov

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Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years' experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

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