Overview

"Despite rapidly expanding interest in potential applications of surrogate tissue analysis and intense competition to identify and validate biomarkers in appropriate surrogate tissues, very few peer reviewed publications describing the use of this approach have appeared in the scientific press. One of the first publications on this topic, Surrogate Tissue Analysis: Genomic, Proteomic, and Metabolomic Approaches describes initial applications and considerations for ""omic"" technologies in the field of surrogate tissue analysis. Highlighting important issues to consider when conducting profiling studies to identify novel biomarkers, the first section covers transcriptional approaches in surrogate tissues. It provides a review of important issues in peripheral blood profiling, summarizes results achieved when evaluations of various blood preparation platforms are used for the purpose of transcriptional profiling, and covers the relatively novel application of transcriptional profiling in neurological and oncological disease settings. The second section focuses on proteomic and protein-based methods for identifying markers in surrogate tissues, highlighting immunoassay and mass-spectrometry approaches for assessment of proteins in serum and other fluids, with a focus on the implications of protein-based biomarkers for detecting and monitoring early stages of cancer. The third section explores metabolomic approaches along with other novel molecular screens that can be applied in surrogate tissues to find biomarkers, and examines in detail the rapid development of metabolomics into a powerful technique for biomarker identification. The authors conclude with coverage of regulatory considerations, economic impact, and pan-omic strategies which will undoubtedly impact surrogate tissue analysis in the future. They explore current concepts in pan-omic approaches during drug development where a compendium of data generated by multiple profiling approaches are assessed and evaluated with its impact on the field of systems biology. The last chapter rounds out the coverage with a brief look ahead towards future analytical issues that will likely arise in the field of surrogate tissue analysis. The book is both an introduction to the various ""omic"" technologies in this young field and a fundamental reference for scientists interested in identifying biomarkers in surrogate tissues."

Full Product Details

Author:   Michael E. Burczynski (Biomarker Laboratory, Collegeville, PA, USA) ,  John C. Rockett (North Bend, Washington, USA) ,  Nigel Waters (Astrazeneca Pharmaceuticals, United Kingdom) ,  John C. Rockett (North Bend, Washington, USA)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Dimensions:   Width: 15.60cm , Height: 2.30cm , Length: 23.40cm
Weight:   0.589kg
ISBN:  

9780849328404

ISBN 10:   0849328403
Pages:   332
Publication Date:   10 November 2005
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

INTRODUCTION TO SURROGATE TISSUE ANALYSIS. Introduction to Surrogate Tissue Analysis. GENOMIC APPROACHES. Impact of Sample Handling and Preparation on Gene Signatures as Exemplified for Transcriptome Analysis of Peripheral Blood. Blood Genomic Fingerprints of Brain Diseases. Transcriptional Profiling of Peripheral Blood in Oncology. Blood-Derived Transcriptomic Profiles as a Means to Monitor Levels of Toxicant Exposure and the Effects of Toxicants on Inaccessible Target Tissues. Spermatozoal RNAs as Surrogate Markers of Paternal Exposure. PROTEOMIC APPROACHES. Proteomic Analysis of Surrogate Tissues: Mass Spectrometry-Based Profiling of the Circulatory Proteome for Cancer Detection and Stratification. Lymphocyte Integrins: Potential Surrogate Biomarkers for Evaluation of Endometrial Receptivity. Nipple Aspirate Fluid to Diagnose Breast Cancer and Monitor Response to Treatment. METABOLOMICS AND OTHER APPROACHES. Metabonomics: Metabolic Profiling and Pattern Recognition Analysis of Body Fluids and Tissues for Characterization of Drug Toxicity and Disease Diagnosis. Comprehensive Metabolomic Profiling of Serum and Cerebrospinal Fluid: Understanding Disease, Human Variability, and Toxicity. Lipidomic Analysis of Plasma and Tissues: Lipid-Derived Mediators of Inflammation and Markers of Disease. Molecular Detection and Characterization of Circulating Tumor Cells and Micrometastases in Solid Tumors. Methylation Profiling of Tumor Cells and Tumor DNA in Blood, Urine, and Body Fluids for Cancer Detection and Monitoring. FUTURE CONSIDERATIONS FOR SURROGATE TISSUE PROFILING. Regulatory and Technical Challenges in Incorporating Surrogate Tissue Profiling Strategies into Clinical Development Programs. Considerations in the Economic Assessment of the Value of Molecular Profiling. The Impact and Challenges of Pan-Omic Approaches in Pharmaceutical Discovery and Development. Current and Future Aspects of Surrogate Tissue Analysis. References.

Reviews

"""In summary, the editors and their coauthors have done a very creditable job of expressing the breadth of the field of surrogate tissue analysis and provide many concrete examples of techniques for analysis. The treatment of this arising technology is at once learned and practical with examples of success and of the need for further research. The chapters at the end with perspective setting essays on economic and regulatory needs may help some not accustomed to these issues to understand the issues. However, the lesson to take away from this text is the great possibilities that exist to sharpen our view of biology and of medicine in particular. Future progress will involve refinement of the methods described here. There are methods that will not stand the test of time but taken as a whole, this book should be widely read. Every chapter has extensive bibliographic references and should serve as entrée into the literature even if the specific examples are of only passing interest. The editors and their coauthors are to be commended in providing a very thorough and interesting sampling of the methods being developed in the field."" Sid Aaron, Sid Aaron Consultants, LLC, in the International Journal of Toxicology, May 2006"

In summary, the editors and their coauthors have done a very creditable job of expressing the breadth of the field of surrogate tissue analysis and provide many concrete examples of techniques for analysis. The treatment of this arising technology is at once learned and practical with examples of success and of the need for further research. The chapters at the end with perspective setting essays on economic and regulatory needs may help some not accustomed to these issues to understand the issues. However, the lesson to take away from this text is the great possibilities that exist to sharpen our view of biology and of medicine in particular. Future progress will involve refinement of the methods described here. There are methods that will not stand the test of time but taken as a whole, this book should be widely read. Every chapter has extensive bibliographic references and should serve as entree into the literature even if the specific examples are of only passing interest. The editors and their coauthors are to be commended in providing a very thorough and interesting sampling of the methods being developed in the field. Sid Aaron, Sid Aaron Consultants, LLC, in the International Journal of Toxicology, May 2006

Author Information

Michael E. Burczynski, John C. Rockett

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