Overview

This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Genome Editing in Animals: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field. 

Full Product Details

Author:   Izuho Hatada
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2637
Weight:   0.969kg
ISBN:  

9781071630150

ISBN 10:   1071630156
Pages:   399
Publication Date:   12 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Construction and Evaluation of Zinc Finger Nucleases Hiroshi Ochiai and Takashi Yamamoto   2. Updated overview of TALEN construction systems Tetsushi Sakuma and Takashi Yamamoto     3. CRISPR/Cas9 Izuho Hatada, Sumiyo Morita, and Takuro Horii   4 SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System Shohei Maruyama, Takashi Kusakabe, Xinyi Zou, Yoshiaki Kobayashi, Yoshimasa Asano, Qingbo S. Wang, and Kumiko Ui-Tei   5. Utilizing large functional and population genomics resources for CRISPR/Cas perturbation experiment design Qingbo S. Wang, Kumiko Ui-Tei   6. Generation of genome-edited mice by cytoplasmic injection of CRISPR-Cas9 RNA Takuro Horii and Izuho Hatada   7. Gene targeting in mouse embryonic stem cells via CRISPR/Cas9 ribonucleoprotein (RNP) mediated genome editing. Manabu Ozawa, Chihiro Emori, and Masahito Ikawa   8. Generation of Knock-in Mouse by Genome Editing Wataru Fujii   9. Introduction of genetic mutations into mice by Base Editor and Target-AID Hiroki Sasaguri   10. Genome editing in mouse and rat by electroporation Takehito Kaneko   11. Generation of floxed mice by sequential electroporation Takuro Horii, Ryosuke Kobayashi, and Izuho Hatada   12. Efficient detection of flox mice using in vitro Cre recombination Ryosuke Kobayashi, Takuro Horii, and Izuho Hatada   13. VCre/VloxP and SCre/SloxP as reliable site-specific recombination systems for genome engineering Manabu Nakayama   14. Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-phase in Mouse Zygotes Takaya Abe, Ken-ichi Inoue, and Hiroshi Kiyonari   15. Genome editing of murine liver hepatocytes by AAV vector-mediated expression of Cas9 in vivo Yuji Kashiwakura and Tsukasa Ohmori   16. Non-Viral Ex Vivo Genome-Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9 Hiromasa Hara1, Natsagdorj Munkh-Erdene, Suvd Byambaa, and Yutaka Hanazono   17. Genome editing of rat Takehito Kaneko   18. A simple and efficient method for generating KO rats using in vitro fertilized oocytes Kohtaro Morita, Arata Honda, and Masahide Asano   19. Editing the Genome of the Golden Hamster (Mesocricetus auratus) Michiko Hirose, Toshiko Tomishima, and Atsuo Ogura   20. Gene targeting in rabbits: single-step generation of knockout rabbits by microinjection of CRISPR/Cas9 plasmids Arata Honda   21. Genome Editing of Pig Masahito Watanabe and Hiroshi Nagashima   22. GEEP method: an optimized electroporation-mediated gene-editing approach for establishment of knockout pig lines Fuminori Tanihara, Maki Hirata, and Takeshige Otoi   23. Genome editing mediated by primordial germ cell in chicken Jae Yong Han and Hong Jo Lee   24. CRISPR–Cas9-mediated genome modifications in zebrafish Yusuke Kamachi and Atsuo Kawahara   25. Genome Editing of Medaka Rie Hara, Satoshi Ansai, and Masato Kinoshita   26. CRISPR-Cas9 based functional analysis in amphibians; Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl Miyuki Suzuki, Midori Iida, Toshinori Hayashi, and Ken-ichi T Suzuki   27. Genome Editing of Silkworms Takuya Tsubota, Hiroki Sakai, and Hideki Sezutsu   28. Improved genome editing in the ascidian Ciona with CRISPR/Cas9 and TALEN Yasunori Sasakura and Takeo Horie   29. Genome editing of C. elegans Takuma Sugi  

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