Overview

Our ability to manipulate the genome of living organisms for the elucidation of gene function in vivo has improved dramatically over the last decade. Genetic Manipulation of Receptor Expression and Function provides a comprehensive review of the principles and applications for genetic manipulation of receptor expression and function. By providing the necessary conceptual framework of transgenic and knockout technologies, Genetic Manipulation of Receptor Expression and Function offers a concise and easily accessible analysis for researchers. The material reviews the numerous advances in the field of transgenic technology allowing researchers to alter expression and function in the mouse genome. This volume also: * Provides a roadmap to transgenic and knockout methodology * Includes a detailed explanation of the differences between embryonic stem cells and fertilized zygotes * Clarifies the use of a DNA construct to make a transgenic mouse and a targeting vector for a knockout * Reviews the considerable progress in receptor research * Covers rapidly changing aspects in transgenic mouse technology (such as the generation of transgenic mice using large DNA fragments, the role of modifier genes, the importance of behavioral testing in analyzing disease phenotypes, and the growing role of antisense techniques in studies of gene function) Receptor biochemists, neuroscientists, molecular biologists, cell biologists, and molecular geneticists will find Genetic Manipulation of Receptor Expression and Function a useful guide.

Full Product Details

Author:   D Accili
Publisher:   John Wiley & Sons Inc
Imprint:   John Wiley & Sons Inc
Dimensions:   Width: 18.50cm , Height: 2.00cm , Length: 26.00cm
Weight:   0.680kg
ISBN:  

9780471350576

ISBN 10:   0471350575
Pages:   256
Publication Date:   13 April 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Transgenic Rescue of Mutant Phenotypes Using Large DNA Fragments (S. Camper & T. Saunders). Behavioral Characterization of Transgenic and Knockout Mice (J. Crawley). Genetic Analysis of Receptor Phenotypes (S. Bultman, et al.). Immortalization of Cell Lines from Transgenic and Knockout Mice by Viral Transformation (J. Chou). The VP16-Dependent Binary System for Inducible Gene Expression in Transgenic Mice (C. Kappen). Gene-Targeting Strategies in the Analysis of Alcohol and Anesthetic Mechanisms (G. Homanics). In Vivo Functions of the c-Met Gene: (HGF/SF Receptor) (S. Giordano & P. Comoglio). Tissue-Specific Knockouts of Insulin Receptor Tyrosine Kinase (R. Kulkarni, et al.). Targeted Dominant Negative Mutations (W. LaRochelle, et al.). Toxin-Mediated Targeted Cell Ablation In Vivo (J. Drago & J. Wong). Antisense RNA-Mediated Inhibition of Gene Expression (H. Wang, et al.). Delivery of Antisense Oligonucleotides to the Brain to Analyze Receptor Function (S. Kaur & I. Creese). Index.

Reviews

Geneticists and molecular biologists provide a conceptual framework of transgenic and knockout technologies, reviewing a variety of modern advances in the field... ( SciTech Book News , Vol. 24, No. 4, December 2000) <p> . ..an exhaustive description of the different technologies...provides useful and specific information and possible applications to this field... (Cell Biology International)

Author Information

Domenico Accili is the author of Genetic Manipulation of Receptor Expression and Function, published by Wiley.

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