|
|
|||
|
||||
OverviewThe book is composed of detailed principles, methods and findings of genetic engineering of colour variants of Aequorea victoria green fluorescent protein (GFP) for thermo-stability, refolding kinetics and pH-sensitivity. In addition, principles and methods to engineer the GFP to develop its destabilized version with a potential to study transient expression of cytoplasmic proteins are meticulously presented. The GFP was also used as a model substrate to elucidate the possible chaperone activity of bacterial initiation factor IF2. Of particular interest is the developed methodology, used throughout the study, to read the fluorescence of the protein in real time using RT-PCR machine while treating the sample at temperature of interest (25-99°C) for the intended period. Such method has never been developed and used before. Much more detailed, handy and innovative methodologies are addressed in the text. Thus, the book, which was forwarded by Prof. Chemeda Fininsa, is intended for graduate students, academicians, beginner and advanced researchers in the area of genetic engineering and biotechnology. Full Product DetailsAuthor: Feto Naser AliyePublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.90cm , Length: 22.90cm Weight: 0.227kg ISBN: 9783843357876ISBN 10: 3843357870 Pages: 148 Publication Date: 29 November 2012 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |