Overview

Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation.

Full Product Details

Author:   Shao-Yao Ying
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2003 ed.
Volume:   221
Dimensions:   Width: 15.50cm , Height: 2.40cm , Length: 23.50cm
Weight:   0.758kg
ISBN:  

9781588290663

ISBN 10:   1588290662
Pages:   338
Publication Date:   19 February 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Awaiting stock  
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Table of Contents

Complementary DNA Libraries.- Rapid Amplification of cDNA Ends.- cDNA Generation on Paramagnetic Beads.- Construction of a Normalized cDNA Library by mRN-cDNA Hybridization and Subtraction.- Amplification of cDNA Ends Using PCR Suppression Effect and Step-Out PCR.- Use of Inverse PCR to Clone cDNA Ends.- Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction.- Construction of a Full-Length Enriched and a 5?-End Enriched cDNA Library Using the Oligo-Capping Method.- cDNA Library Construction Using In Vitro Transcriptional Amplification.- Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue.- Single-Cell cDNA Library Construction Using Cycling aRNA Amplification.- mRNA/cDNA Library Construction Using RNA—Polymerase Cycling Reaction.- Quality Assessment of cDNA Libraries.- Assessment of the Quality of mRNA Libraries by Agarose Gel Electrophoresis.- PACS RT-PCR.- Single-Cell mRNA Library Analysis by Northern Blot Hybridization.- Generation of cDNA Libraries for Profiling Gene Expression of Given Tissues or Cells.- Screening Poly [dA/dT(?)] cDNA for Gene Identification.- Generation of Longer cDNA Fragments from SAGE Tags for Gene Identification.- Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes.- Subtractive Hybridization for the Identification of Differentially Expressed Genes Using Uraci-DNA Glycosylase and Mung-Bean Nuclease.- Subtractive Cloning of Differential Genes Using RNA-PCR.- Strategy for Construction of a cDNA Encoding a Repetitive Amino Acid Sequence.- Preparing Lambda Libraries for Expression of Proteins in Prokaryotes or Eukaryotes.- Peptide Library Construction from RNA-PCR-Derived RNAs.- Identifying Interacting Proteins in an Escherichiacoli-Based Two-Hybrid System.- Future Perspectives.

Reviews

the ready-for-use protocols are excellent A good buy. Cell Biochem Funct

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