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OverviewIn this fully updated and revised 2nd edition of Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application. Full Product DetailsAuthor: Jeffrey R. MorganPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 2nd Revised edition Volume: v. 69 Dimensions: Width: 15.20cm , Height: 3.40cm , Length: 22.90cm Weight: 0.958kg ISBN: 9780896037236ISBN 10: 0896037231 Pages: 544 Publication Date: 15 October 2001 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: Out of stock  Table of ContentsPoly-l-Lysine-Based Gene Delivery Systems: Synthesis, Purification, and Application Charles P. Lollo, Mariusz G. Banaszczyk, Patricia M. Mullen, Christopher C. Coffin, Dongpei Wu, Alison T. Carlo, Donna L. Bassett, Erin K. Gouveia, and Dennis J. Carlo Targeted Gene Transfer to Liver Using Protein-DNA Complexes Catherine H. Wu, Cherie M. Walton, and George Y. Wu Receptor-Directed Molecular Conjugates for Gene Transfer Assem G. Ziady and Pamela B. Davis Gene Transfer into Muscle by Electroporation in Vivo Jun-ichi Miyazaki and Hiroyuki Aihara Viral Liposomes: Preparation and Use Yasufumi Kaneda LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery Yadi Tan, Mark Whitmore, Song Li, Peter Frederik, and Leaf Huang Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use Klaus Schughart and Ulla B. Rasmussen Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts Olga A. Igoucheva and Kyonggeun Yoon Regulated Expression of Plasmid-Based Gene Therapies Ronald V. Abruzzese, Fiona C. MacLaughlin, Louis C. Smith, and Jeffrey L. Nordstrom Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery Lina Prasmickaite, Anders Hogset, and Kristian Berg Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun Chia-Feng Kuo, Jeng Hwan Wang, and Ning-Sun Yang Preparation of Pseudotyped Retroviral Vector Hong Yu Quantitative Measurement of the Concentration of Active Recombinant Retrovirus Stelios T. Andreadis and Jeffrey R. Morgan Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use Jarmo Wahlfors and Richard A. Morgan Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells William P. Swaney, Enrico M. Novelli, Alfred B. Bahnson, and John A. Barranger Genetically Modified Skin Substitutes: Preparation and Use Karen E. Hamoen, Gulsun Erdag, Jennifer L. Cusick, Hinne A. Rakhorst, and Jeffrey R. Morgan Bioartificial Muscles in Gene Therapy Courtney Powell, Janet Shansky, Michael Del Tatto, and Herman H. Vandenburgh Cytokine Gene-Modified Cell-Based Cancer Vaccines R. Todd Reilly, Jean-Pascal H. Machiels, Leisha A. Emens, and Elizabeth M. Jaffee HIV-Based Vectors: Preparation and Use Antonia Follenzi and Luigi Naldini Packaging Cell System for Lentivirus Vectors: Preparation and Use Narasimhachar Srinivasakumar Lentiviral Vectors: Preparation and Use Lung-Ji Chang and Anne-Kathrin Zaiss Simian Foamy Virus Vectors: Preparation and Use Jeonghae Park and Ayalew Mergia Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use Michael A. Curran and Garry P. Nolan Lentivirus Vector Based on Simian Immunodeficiency Virus: Development and Use Klaus Uberla Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus: Production and Use Akihiro Iida and Mamoru Hasegawa Preparation of Helper-Dependent Adenoviral Vectors Philip Ng, Robin J. Parks, and Frank L. Graham Construction of First-Generation Adenoviral Vectors Philip Ng and Frank L. Graham Preparation of Ovine Adenovirus Vectors Peter Loser, Daniel Kumin, Moritz Hillgenberg, Gerald W. Both, and Christian Hofmann Highly Purified Recombinant Adeno-Associated Virus Vectors: Preparation and Quantitation Bruce Schnepp and K. Reed Clark High-Titer Stocks of Adeno-Associated Virus from Replicating Amplicons and Herpes Vectors Ajay R. Mistry, Mahesh De Alwis, Elisabeth Feudner, Robin R. Ali, and Adrian J. Thrasher Herpes Simplex Virus/Adeno-Associated Virus Hybrid Vectors for Gene Transfer to Neurons: Preparation and Use Lauren C. Costantini, Cornel Fraefel, Xandra O. Breakefield, and Ole Isacson Development of Replication-Defective Herpes Simplex Virus Vectors William F. Goins, David M. Krisky, Darren P. Wolfe, David J. Fink, and Joseph C. GloriosoReviewsEach protocol is written in a standard format, consisting of introduction, materials, methods, notes, and references. The introductions vary in length and detail. Many of them are illustrated with tables, charts, schematics, and/or photographs. The materials and methods sections are quite detailed, and there are many notes clarifying steps or outlining potential problems. Gene Therapy Protocols is recommended for university, hospital, and corporate libraries serving researchers who are working on gene therapies. - E-Streams From Reviews of the First Edition: extremely impressive a must, even for researchers who are already familiar with gene transfer techniques - Molecular Medicine Today succeeds admirably - Genetical Research Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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